Rabbit anti-Human Cleaved-Caspase-8 Polyclonal Antibody | anti-CASP8 antibody

Cleaved-Caspase-8 (D384) Polyclonal Antibody

Cat. Num. AAA28833

• Gene Names: CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Synonyms: Cleaved-Caspase-8; Polyclonal Antibody; Cleaved-Caspase-8 (D384) Polyclonal Antibody; CASP8; MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; anti-CASP8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.

• Applicable Applications for anti-CASP8 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
• Application Notes: WB: 1:500-2000; IF 1:50-300; IHC: 1:50-300
• Tissue Location: Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle
• Notes: ECO:0000303|PubMed:9931493, ECO:0000312|HGNC: HGNC:1509
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistchemistry)

(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistochemistry)

(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin)

(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin)

(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin)

(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

WB (Western Blot)

(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

Related Product Information for anti-CASP8 antibody

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (By similarity). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death (PubMed:23516580, PubMed:8681376, PubMed:8681377, PubMed:9006941, PubMed:9184224, PubMed:8962078). Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (PubMed:8962078, PubMed:9006941). Binding to the adapter molecule FADD recruits it to either receptor TNFRSF6/FAS mediated or TNFRSF1A (PubMed:8681376, PubMed:8681377). The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation (PubMed:9184224). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (PubMed:9184224). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (PubMed:9184224). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-324', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (PubMed:31827280, PubMed:31827281). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-D (GSDMD): GSDMD cleavage promoting release of the N-terminal moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (By similarity). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (By similarity). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (By similarity). May participate in the Granzyme B (GZMB) cell death pathways (PubMed:8755496). Cleaves PARP1 (PubMed:8681376).

NCBI and Uniprot Product Information

• NCBI GI #: 122056474
• NCBI GeneID: 841
• NCBI Accession #: NP_001073593.1
• NCBI GenBank Nucleotide #: NM_001080124.1
• UniProt Accession #: Q14790; O14676; Q14791; Q14792; Q14793; Q14794; Q14795; Q14796; Q15780; Q15806; Q53TT5; Q8TDI1
• Molecular Weight: 55,391 Da
• NCBI Official Full Name: caspase-8 isoform C
• NCBI Official Synonym Full Names: caspase 8, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP8
• NCBI Official Synonym Symbols: CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
• NCBI Protein Information: caspase-8; FADD-like ICE; MACH-alpha-1/2/3 protein; apoptotic protease Mch-5; MACH-beta-1/2/3/4 protein; apoptotic cysteine protease; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; FADD-homologous ICE/CED-3-like protease; caspase 8, apopto
• UniProt Protein Name: Caspase-8
• UniProt Gene Name: CASP8
• UniProt Synonym Gene Names: MCH5; CASP-8; FLICE; MACH
• UniProt Entry Name: CASP8_HUMAN