FCM/FACS (Flow Cytometry)
(Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5 ug in 100 uL1X PBS containing 0.5% BSA(green).)
Rabbit anti-Rat, Cow CLEC 4E Polyclonal Antibody | anti-CLEC4E antibody
CLEC 4E Polyclonal Antibody
Gene Names
CLEC4E; MINCLE; CLECSF9
Reactivity
Rat, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CLEC 4E, Antibody; CLEC 4E Polyclonal Antibody; C-type lectin domain family 4 member E; C-type lectin superfamily member 9; Macrophage-inducible C-type lectin; MINCLE; CLEC4E; anti-CLEC4E antibody
Host
Rabbit
Reactivity
Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-CLEC4E antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Notes
ECO:0000303|PubMed:24101491, ECO:0000312|HGNC: HGNC:14555
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5 ug in 100 uL1X PBS containing 0.5% BSA(green).)
FCM/FACS (Flow Cytometry)
(Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5 ug in 100 uL1X PBS containing 0.5% BSA(green).)
FCM/FACS (Flow Cytometry)
(Black line : Positive blank control (Hela); Negative blank control (A431) Green line : Primary Antibody (Rabbit Anti-CLEC 4E antibody (bs-8541R) ) Orange line?Isotype Control Antibody (Rabbit IgG) .Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488) Hela(Positive)and A431(Negative control)cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Antibody(bs-8541R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).)
FCM/FACS (Flow Cytometry)
(Black line : Positive blank control (Hela); Negative blank control (A431) Green line : Primary Antibody (Rabbit Anti-CLEC 4E antibody (bs-8541R) ) Orange line?Isotype Control Antibody (Rabbit IgG) .Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488) Hela(Positive)and A431(Negative control)cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Antibody(bs-8541R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,073 Da
NCBI Official Full Name
C-type lectin domain family 4 member E
NCBI Official Synonym Full Names
C-type lectin domain family 4, member E
NCBI Official Symbol
CLEC4E
NCBI Official Synonym Symbols
MINCLE; CLECSF9
NCBI Protein Information
C-type lectin domain family 4 member E; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; C-type lectin superfamily member 9; macrophage-inducible C-type lectin
UniProt Protein Name
C-type lectin domain family 4 member E
UniProt Gene Name
CLEC4E
UniProt Synonym Gene Names
MINCLE1 PublicationManual assertion based on opinion iniRef.10
Similar Products
Product Notes
The CLEC4E clec4e (Catalog #AAA292154) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLEC 4E Polyclonal Antibody reacts with Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CLEC 4E can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CLEC4E clec4e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC 4E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.