IHC (Immunohistochemisry)
(CLEC4E/MINCLE Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.)
Rabbit anti-Mouse, Human CLEC4E/MINCLE Polyclonal Antibody | anti-CLEC4E antibody
CLEC4E/MINCLE Rabbit anti-Human Polyclonal Antibody
Gene Names
CLEC4E; MINCLE; CLECSF9
Reactivity
Mouse, Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Protein G purified
Synonyms
CLEC4E/MINCLE, Antibody; CLEC4E/MINCLE Rabbit anti-Human Polyclonal Antibody; IHC-plus CLEC4E/MINCLE Antibody; CLEC4E; CLECSF9; MINCLE; anti-CLEC4E antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human CLEC4E/MINCLE
Purity/Purification
Protein G purified
Form/Format
PBS, pH7.4, 0.03% Proclin 300, 50% glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CLEC4E antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA
Target
Human CLEC4E/MINCLE
Immunogen
Recombinant human C-type lectin domain family 4 member E protein.
Conjugation
Unconjugated
Preparation and Storage
Short term: store at -20 degree C. Long term: store at -80 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemisry)
(CLEC4E/MINCLE Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.)
IHC (Immunohistochemisry)
(CLEC4E/MINCLE Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.)
WB (Western Blot)
(CLEC4E/MINCLE Antibody-Western blot. All lanes: CLEC4E antibody at 0.3 ug/ml+ Mouse spleen tissue Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 25 kDa. Observed band size: 25 kDa.)
WB (Western Blot)
(CLEC4E/MINCLE Antibody-Western blot. All lanes: CLEC4E antibody at 0.3 ug/ml+ Mouse spleen tissue Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 25 kDa. Observed band size: 25 kDa.)
ICC (Immunocytochemistry)
(CLEC4E/MINCLE Antibody-Immunofluorescence staining of U251 cells with CLEC4E Antibody at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
ICC (Immunocytochemistry)
(CLEC4E/MINCLE Antibody-Immunofluorescence staining of U251 cells with CLEC4E Antibody at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Related Product Information for anti-CLEC4E antibody
MINCLE antibody is an unconjugated rabbit polyclonal antibody to MINCLE (CLEC4E) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,073 Da
NCBI Official Full Name
C-type lectin domain family 4 member E
NCBI Official Synonym Full Names
C-type lectin domain family 4, member E
NCBI Official Symbol
CLEC4E
NCBI Official Synonym Symbols
MINCLE; CLECSF9
NCBI Protein Information
C-type lectin domain family 4 member E; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; C-type lectin superfamily member 9; macrophage-inducible C-type lectin
UniProt Protein Name
C-type lectin domain family 4 member E
UniProt Gene Name
CLEC4E
UniProt Synonym Gene Names
MINCLE1 PublicationManual assertion based on opinion iniRef.10
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Product Notes
The CLEC4E clec4e (Catalog #AAA162867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLEC4E/MINCLE Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLEC4E/MINCLE can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the CLEC4E clec4e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC4E/MINCLE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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