Rabbit CLPX Polyclonal Antibody | anti-CLPX antibody

CLPX Antibody (C-term)

Cat. Num. AAA285094

• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CLPX, Antibody; CLPX Antibody (C-term); ATP-dependent Clp protease ATP-binding subunit clpX-like; mitochondrial; CLPX; anti-CLPX antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CLPX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 449-477 amino acids from the C-terminal region of human CLPX.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 633

• Applicable Applications for anti-CLPX antibody: ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
• Antigen Region: 449-477
• Cellular location: Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid
• Tissue location: Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain,placenta, lung, kidney and pancreas.
• Precautions: CLPX Antibody (C-term)is for research use only
• Function: Name CPX FunctionATP-dependent specificity comp onent of the Clp protease complex. Hydrolyzes ATP.Targets specific substrates for degradation by the Clp complex (PubMed: , PubMed:22710082). Can perform chaperone functions in the absence of CLPP. Enhances the DNA-binding activity of TFAM and is required for maintaining anormal mitochondrial nucleoid structure (PubMed: ). ATP-dependent unfoldase that stimulates theincorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase,thereby activating 5-aminolevulinate (ALA)synthesis, the first step in heme biosynthesis.Important for efficient erythropoiesis through upregulation of heme biosynthesis(PubMed: ).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(CLPX Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(CLPX antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLPX antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of lysate from human heart tissue lysate, using CLPX Antibody (C-term). AAA285094 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

Related Product Information for anti-CLPX antibody

ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP (By similarity).

Product Categories/Family for anti-CLPX antibody

Cell Biology; Metabolism; Signal transduction

References

Martin, A., et al. Mol. Cell 29(4):441-450(2008) Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : Kang, S.G., et al. J. Biol. Chem. 280(42):35424-35432(2005) Kang, S.G., et al. J. Biol. Chem. 277(23):21095-21102(2002)

NCBI and Uniprot Product Information

• NCBI GI #: 7242140
• NCBI GeneID: 10845
• NCBI Accession #: NP_006651.2
• NCBI GenBank Nucleotide #: NM_006660.3
• UniProt Accession #: O76031; Q9H4D9; A1L428; A8K8F1; B9EGI8
• NCBI Official Full Name: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
• NCBI Official Synonym Full Names: caseinolytic mitochondrial matrix peptidase chaperone subunit
• NCBI Official Symbol: CLPX
• NCBI Protein Information: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
• UniProt Protein Name: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
• UniProt Gene Name: CLPX
• UniProt Entry Name: CLPX_HUMAN