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FCM/FACS (Flow Cytometry)
(CMGA Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CMGA Polyclonal Antibody | anti-CHGA antibody
CMGA Antibody (C-term)
Gene Names
CHGA; CGA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CMGA, Antibody; CMGA Antibody (C-term); Chromogranin-A; CgA; Pituitary secretory protein I; SP-I; Vasostatin-1; Vasostatin I; Vasostatin-2; Vasostatin II; EA-92; ES-43; Pancreastatin; SS-18; WA-8; WE-14; LF-19; AL-11; GV-19; GR-44; ER-37; CHGA; anti-CHGA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CMGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-404 amino acids from the C-terminal region of human CMGA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
376-404
Sequence Length
457
Applicable Applications for anti-CHGA antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CMGA Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CMGA Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CMGA Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CMGA Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(CMGA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CMGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(CMGA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CMGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(CMGA Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CMGA antibody detected the CMGA protein (arrow).)
WB (Western Blot)
(CMGA Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CMGA antibody detected the CMGA protein (arrow).)
Related Product Information for anti-CHGA antibody
The protein encoded by this gene is a member of the
chromogranin/secretogranin family of neuroendocrine secretory
proteins. It is found in secretory vesicles of neurons and
endocrine cells. This gene product is a precursor to three
biologically active peptides; vasostatin, pancreastatin, and
parastatin. These peptides act as autocrine or paracrine negative
modulators of the neuroendocrine system. Other peptides, including
chromostatin, beta-granin, WE-14 and GE-25, are also derived from
the full-length protein. However, biological activities for these
molecules have not been shown.
chromogranin/secretogranin family of neuroendocrine secretory
proteins. It is found in secretory vesicles of neurons and
endocrine cells. This gene product is a precursor to three
biologically active peptides; vasostatin, pancreastatin, and
parastatin. These peptides act as autocrine or paracrine negative
modulators of the neuroendocrine system. Other peptides, including
chromostatin, beta-granin, WE-14 and GE-25, are also derived from
the full-length protein. However, biological activities for these
molecules have not been shown.
Product Categories/Family for anti-CHGA antibody
References
Ezzi, S.A., et al. J. Neurochem. 115(5):1102-1111(2010)
Ma, Z., et al. J. Urol. 184(3):1182-1188(2010)
Ramella, R., et al. J. Cell. Biochem. 110(1):70-79(2010)
Dag, E., et al. Peptides 31(5):932-937(2010)
Xie, Y.Q., et al. Zhonghua Xin Xue Guan Bing Za Zhi 37(12):1081-1084(2009)
Ma, Z., et al. J. Urol. 184(3):1182-1188(2010)
Ramella, R., et al. J. Cell. Biochem. 110(1):70-79(2010)
Dag, E., et al. Peptides 31(5):932-937(2010)
Xie, Y.Q., et al. Zhonghua Xin Xue Guan Bing Za Zhi 37(12):1081-1084(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50688
NCBI Official Full Name
chromogranin-A isoform 1 preproprotein
NCBI Official Synonym Full Names
chromogranin A
NCBI Official Symbol
CHGA
NCBI Official Synonym Symbols
CGA
NCBI Protein Information
chromogranin-A
UniProt Protein Name
Chromogranin-A
UniProt Gene Name
CHGA
UniProt Synonym Gene Names
CgA; SP-I
UniProt Entry Name
CMGA_HUMAN
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Product Notes
The CHGA chga (Catalog #AAA285162) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 376-404. The CMGA Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CMGA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CHGA chga for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CMGA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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