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Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit CNPase Polyclonal Antibody | anti-CNPase antibody
CNPase Antibody
Gene Names
CNP; CNP1
Reactivity
Human, Mouse, MonkeyPredicted Reactivity: Pig (83%), Bovine (83%), Horse (83%), Sheep (83%), Dog (83%)
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
CNPase, Antibody; CNPase Antibody; 2''; 2'3' cyclic nucleotide 3' phosphodiesterase; 3''-cyclic-nucleotide 3''-phosphodiesterase; CN37_HUMAN; CNP 1; CNP; CNP1; CNPase; anti-CNPase antibody
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Predicted Reactivity: Pig (83%), Bovine (83%), Horse (83%), Sheep (83%), Dog (83%)
Predicted Reactivity: Pig (83%), Bovine (83%), Horse (83%), Sheep (83%), Dog (83%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
CNPase Antibody detects endogenous levels of total CNPase.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CNPase antibody
ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CNPase, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Exists as monomers and homodimers.
Similarity
Belongs to the 2H phosphoesterase superfamily. CNPase family.
Subcellular Location
Membrane>Lipid-anchor. Melanosome.
Note: Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Note: Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from VERO, using CNPase Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from VERO, using CNPase Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-CNPase antibody
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.
Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 2'-phosphate.
Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 2'-phosphate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
421
NCBI Official Full Name
2',3'-cyclic-nucleotide 3'-phosphodiesterase
NCBI Official Synonym Full Names
2',3'-cyclic nucleotide 3' phosphodiesterase
NCBI Official Symbol
CNP
NCBI Official Synonym Symbols
CNP1
NCBI Protein Information
2',3'-cyclic-nucleotide 3'-phosphodiesterase; CNPase; 2', 3' cyclic nucleotide 3' phosphohydrolase
UniProt Protein Name
2',3'-cyclic-nucleotide 3'-phosphodiesterase
UniProt Gene Name
CNP
UniProt Synonym Gene Names
CNP; CNPase
UniProt Entry Name
CN37_HUMAN
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Product Notes
The CNPase cnp (Catalog #AAA329391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CNPase Antibody reacts with Human, Mouse, Monkey Predicted Reactivity: Pig (83%), Bovine (83%), Horse (83%), Sheep (83%), Dog (83%) and may cross-react with other species as described in the data sheet. AAA Biotech's CNPase can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CNPase cnp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNPase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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