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IHC (Immunohistochemistry)
(Anti- Cofilin Picoband antibody, AAA46446, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
Cofilin Polyclonal Antibody | anti-CFL1 antibody
Anti-Cofilin Antibody
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Cofilin, Antibody; Anti-Cofilin Antibody; Cofilin-1; 18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18; cofilin 1 (non-muscle); anti-CFL1 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
166
Applicable Applications for anti-CFL1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin (122-152aa DAIKKKLTGIKHELQANCYEEVKDRCTLAEK), identical to the related mouse and rat sequences.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti- Cofilin Picoband antibody, AAA46446, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti- Cofilin Picoband antibody, AAA46446, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- Cofilin Picoband antibody, AAA46446, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemisry)
(Anti- Cofilin Picoband antibody, AAA46446, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti- Cofilin Picoband antibody, AAA46446, IHC(P)IHC(P): Mouse Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti- Cofilin Picoband antibody, AAA46446, IHC(P)IHC(P): Mouse Intestine Tissue)
WB (Western Blot)
(Anti- Cofilin Picoband antibody, AAA46446, Western blottingAll lanes: Anti Cofilin (AAA46446) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Ovary Tissue Lysate at 50ugLane 4: Mouse Liver Tissue Lysate at 50ugLane 5: Mouse Brain Tissue Lysate at 50ugLane 6: Mouse Lung Tissue Lysate at 50ugLane 7: HELA Whole Cell Lysate at 40ugLane 8: JURKAT Whole Cell Lysate at 40ugLane 9: A549 Whole Cell Lysate at 40ugLane 10: SMMC Whole Cell Lysate at 40ugPredicted bind size: 19KDObserved bind size: 19KD)
WB (Western Blot)
(Anti- Cofilin Picoband antibody, AAA46446, Western blottingAll lanes: Anti Cofilin (AAA46446) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Ovary Tissue Lysate at 50ugLane 4: Mouse Liver Tissue Lysate at 50ugLane 5: Mouse Brain Tissue Lysate at 50ugLane 6: Mouse Lung Tissue Lysate at 50ugLane 7: HELA Whole Cell Lysate at 40ugLane 8: JURKAT Whole Cell Lysate at 40ugLane 9: A549 Whole Cell Lysate at 40ugLane 10: SMMC Whole Cell Lysate at 40ugPredicted bind size: 19KDObserved bind size: 19KD)
Related Product Information for anti-CFL1 antibody
Description: Rabbit IgG polyclonal antibody for Cofilin-1(CFL1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70% sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH, phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.
Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70% sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH, phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.
References
1. Cooper, G. M. and R. E. Hausman. The Cell: A Molecular Approach, 3rd ed. Washington DC: ASM Press 2004 pp.436-440. 2. Ichetovkin I, Han J, Pang KM, Knecht DA, Condeelis JS (April 2000). "Actin filaments are severed by both native and recombinant dictyostelium cofilin but to different extents". Cell Motil. Cytoskeleton 45 (4): 293-306. 3. Lappalainen P, Drubin DG (July 1997). "Cofilin promotes rapid actin filament turnover in vivo".Nature 388 (6637): 78-82.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,502 Da
NCBI Official Full Name
cofilin-1
NCBI Official Synonym Full Names
cofilin 1
NCBI Official Symbol
CFL1
NCBI Official Synonym Symbols
CFL; cofilin; HEL-S-15
NCBI Protein Information
cofilin-1
UniProt Protein Name
Cofilin-1
UniProt Gene Name
CFL1
UniProt Synonym Gene Names
CFL; p18
UniProt Entry Name
COF1_HUMAN
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Product Notes
The CFL1 cfl1 (Catalog #AAA46446) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cofilin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cofilin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CFL1 cfl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cofilin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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