WB (Western Blot)
(Anti-COL4A3 Antibody (N-Term) at 1:1000 dilution + kidney lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 162 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human COL4A3 Polyclonal Antibody | anti-COL4A3 antibody
COL4A3 Antibody (N-Term)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
COL4A3, Antibody; COL4A3 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Collagen alpha-3(IV) chain; Goodpasture antigen; Tumstatin; COL4A3; anti-COL4A3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
This COL4A3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 141-173 amino acids from human COL4A3.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide
Sequence Length
1670
Applicable Applications for anti-COL4A3 antibody
ELISA, WB (Western Blot)
Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Cellular Location
Secreted, extracellular space, extracellular matrix, basement membrane. Note=Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina (BL)
Tissue Location
Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. According to PubMed:8083201, alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. According to PubMed:7523402, alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-COL4A3 Antibody (N-Term) at 1:1000 dilution + kidney lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 162 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-COL4A3 Antibody (N-Term) at 1:1000 dilution + kidney lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 162 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-COL4A3 antibody
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Product Categories/Family for anti-COL4A3 antibody
References
Mariyama M.,et al.J. Biol. Chem. 269:23013-23017(1994). Leinonen A.,et al.Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases. Heidet L.,et al.J. Am. Soc. Nephrol. 12:97-106(2001). Hillier L.W.,et al.Nature 434:724-731(2005). Momota R.,et al.FEBS Lett. 424:11-16(1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
161813
NCBI Official Full Name
collagen alpha-3(IV) chain
NCBI Official Synonym Full Names
collagen type IV alpha 3
NCBI Official Symbol
COL4A3
NCBI Protein Information
collagen alpha-3(IV) chain
UniProt Protein Name
Collagen alpha-3(IV) chain
UniProt Gene Name
COL4A3
UniProt Entry Name
CO4A3_HUMAN
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Product Notes
The COL4A3 col4a3 (Catalog #AAA290633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COL4A3 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COL4A3 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the COL4A3 col4a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL4A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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