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IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COL9A1 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit COL9A1 Polyclonal Antibody | anti-COL9A1 antibody
COL9A1 Antibody (Center)
Gene Names
COL9A1; MED; EDM6; STL4; DJ149L1.1.2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
COL9A1, Antibody; COL9A1 Antibody (Center); Collagen alpha-1(IX) chain; COL9A1; anti-COL9A1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This COL9A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-456 amino acids from the Central region of human COL9A1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
428-456
Sequence Length
328
Applicable Applications for anti-COL9A1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COL9A1 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COL9A1 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-COL9A1 Antibody (Center) in 293 cell line lysates (35ug/lane). COL9A1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-COL9A1 Antibody (Center) in 293 cell line lysates (35ug/lane). COL9A1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(COL9A1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the COL9A1 antibody detected the COL9A1 protein (arrow).)
WB (Western Blot)
(COL9A1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the COL9A1 antibody detected the COL9A1 protein (arrow).)
WB (Western Blot)
(COL9A1 Antibody (Center) western blot analysis in MCF-7 cell line and mouse eyeball,rat eyeball tissue lysates (35ug/lane).This demonstrates the COL9A1 antibody detected the COL9A1 protein (arrow).)
WB (Western Blot)
(COL9A1 Antibody (Center) western blot analysis in MCF-7 cell line and mouse eyeball,rat eyeball tissue lysates (35ug/lane).This demonstrates the COL9A1 antibody detected the COL9A1 protein (arrow).)
Related Product Information for anti-COL9A1 antibody
COL9A1 is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule,and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in the COL9A1 gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects.
Product Categories/Family for anti-COL9A1 antibody
References
Fresquet,M., J. Biol. Chem. 282 (48), 34634-34643 (2007)
Liu,L.Y., Yi Chuan 29 (4), 427-432 (2007)
Van Camp,G., Am. J. Hum. Genet. 79 (3), 449-457 (2006)
Sivakumaran,T.A., J. Assoc. Res. Otolaryngol. 7 (2), 160-172 (2006)
Liu,L.Y., Yi Chuan 29 (4), 427-432 (2007)
Van Camp,G., Am. J. Hum. Genet. 79 (3), 449-457 (2006)
Sivakumaran,T.A., J. Assoc. Res. Otolaryngol. 7 (2), 160-172 (2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
91869
NCBI Official Full Name
collagen alpha-1(IX) chain isoform 1
NCBI Official Synonym Full Names
collagen, type IX, alpha 1
NCBI Official Symbol
COL9A1
NCBI Official Synonym Symbols
MED; EDM6; STL4; DJ149L1.1.2
NCBI Protein Information
collagen alpha-1(IX) chain
UniProt Protein Name
Collagen alpha-1(IX) chain
UniProt Gene Name
COL9A1
UniProt Entry Name
CO9A1_HUMAN
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Product Notes
The COL9A1 col9a1 (Catalog #AAA287577) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 428-456. The COL9A1 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's COL9A1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the COL9A1 col9a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL9A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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