IHC (Immunohiostchemistry)
(AAA328878 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit Collagen X Polyclonal Antibody | anti-COL10A1 antibody
Collagen X Antibody
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
Peptide affinity purification
Synonyms
Collagen X, Antibody; Collagen X Antibody; COAA1_HUMAN; Col10a 1; COL10A1; Collagen alpha 1(X) chain; Collagen alpha-1(X) chain; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia); Collagen type X alpha 1; Collagen X alpha 1 polypeptide; CollagenX; fa66d11; fb10c08; OTTHUMP00000040411; Procollagen type X alpha 1; Schmid metaphyseal chondrodysplasia; wu:fa66d11; wu:fb10c08; anti-COL10A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Collagen X Antibody detects endogenous levels of total Collagen X.
Purity/Purification
Peptide affinity purification
Form/Format
Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
680
Applicable Applications for anti-COL10A1 antibody
ELISA
Immunogen
A synthesized peptide derived from human Collagen X, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20°C for 12 months. Avoid repeated freeze/thaw cycles
IHC (Immunohiostchemistry)
(AAA328878 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328878 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from K562, using Collagen X Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from K562, using Collagen X Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-COL10A1 antibody
Function: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
Post Translational Modifications: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Subcellular Location: Secreted>Extracellular space>Extracellular matrix.
Subunit Structure: Homotrimer.
Post Translational Modifications: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Subcellular Location: Secreted>Extracellular space>Extracellular matrix.
Subunit Structure: Homotrimer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
66kDa
NCBI Official Full Name
collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia)
NCBI Official Synonym Full Names
collagen type X alpha 1 chain
NCBI Official Symbol
COL10A1
NCBI Protein Information
collagen alpha-1(X) chain
UniProt Protein Name
Collagen alpha-1(X) chain
UniProt Gene Name
COL10A1
UniProt Entry Name
COAA1_HUMAN
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Product Notes
The COL10A1 col10a1 (Catalog #AAA328878) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen X Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen X can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the COL10A1 col10a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen X, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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