IHC (Immunohiostchemistry)
(AAA321220 at 1/100 staining human Skeletal muscle tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human Collagen XIIalpha 1 Polyclonal Antibody | anti-COL12A1 antibody
Collagen XIIalpha 1 Antibody
Gene Names
COL12A1; UCMD2; BTHLM2; EDSMYP; COL12A1L; BA209D8.1; DJ234P15.1
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Collagen XIIalpha 1, Antibody; Collagen XIIalpha 1 Antibody; BA209D8.1; COCA1; COL12A1; COL12A1L; Collagen alpha-1(XII) chain; collagen type XII proteoglycan; collagen, type XII, alpha 1; DJ234P15.1; anti-COL12A1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Collagen XIIalpha 1 antibody detects endogenous levels of Collagen XIIalpha 1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
3063
Applicable Applications for anti-COL12A1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
A synthesized peptide derived from human Collagen XIIalpha 1
Subcellular Location
Secreted
Tissue Specificity
Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, kidney and a squamous cell carcinoma cell line. Isoform 1 is also present in the corneal epithelial Bowman's membrane (BM) and the interfibrillar matrix of the corneal stroma, but it is not detected in the limbal BM.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA321220 at 1/100 staining human Skeletal muscle tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA321220 at 1/100 staining human Skeletal muscle tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence)
(AAA321220 staining U-2 OS cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA321220 staining U-2 OS cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-COL12A1 antibody
Description: This gene encodes the alpha chain of type XII collagen, a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen family. Type XII collagen is a homotrimer found in association with type I collagen, an association that is thought to modify the interactions between collagen I fibrils and the surrounding matrix. Two transcript variants encoding different isoforms have been identified for this gene.
Function: Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
Subunit Structure: Trimer of identical chains each containing 190 kDa of non-triple-helical sequences.
Post-translational Modifications: The triple-helical tail is stabilized by disulfide bonds at each end. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. Isoform 1 O-glycosylation; glycosaminoglycan of chondroitin-sulfate type.
Similarity: Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Function: Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
Subunit Structure: Trimer of identical chains each containing 190 kDa of non-triple-helical sequences.
Post-translational Modifications: The triple-helical tail is stabilized by disulfide bonds at each end. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. Isoform 1 O-glycosylation; glycosaminoglycan of chondroitin-sulfate type.
Similarity: Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 333 kDa
Predicted: 334 kDa
Predicted: 334 kDa
NCBI Official Full Name
collagen alpha-1(XII) chain long isoform
NCBI Official Synonym Full Names
collagen type XII alpha 1 chain
NCBI Official Symbol
COL12A1
NCBI Official Synonym Symbols
UCMD2; BTHLM2; EDSMYP; COL12A1L; BA209D8.1; DJ234P15.1
NCBI Protein Information
collagen alpha-1(XII) chain
UniProt Protein Name
Collagen alpha-1(XII) chain
UniProt Gene Name
COL12A1
UniProt Synonym Gene Names
COL12A1L
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Product Notes
The COL12A1 col12a1 (Catalog #AAA321220) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen XIIalpha 1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen XIIalpha 1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the COL12A1 col12a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen XIIalpha 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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