WB (Western Blot)
(Detection of human Cortactin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Cortactin antibody AAA211940 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. Cortactin was also immunoprecipitated by rabbit anti-Cortactin antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
Rabbit anti-Human Cortactin Polyclonal Antibody | anti-CTTN antibody
Rabbit anti-Cortactin Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Cortactin, Antibody; Rabbit anti-Cortactin Antibody, Affinity Purified; EMS1; src substrate cortactin; oncogene EMS1; epididymis secretory sperm binding protein; amplaxin; 1110020L01Rik; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)); Src substrate cortactin; cortactin; anti-CTTN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CTTN antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 450 and 500
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Cortactin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Cortactin antibody AAA211940 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. Cortactin was also immunoprecipitated by rabbit anti-Cortactin antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
WB (Western Blot)
(Detection of human Cortactin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Cortactin antibody AAA211940 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. Cortactin was also immunoprecipitated by rabbit anti-Cortactin antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
IHC (Immunohistochemistry)
(Detection of human Cortactin by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Cortactin (Cat. No. AAA211940) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Cortactin by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Cortactin (Cat. No. AAA211940) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-CTTN antibody
Cortactin has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. The aberrant regulation of cortactin contributes to tumor cell invasion and metastasis [taken from NCBI Entrez Gene (GeneID: 2017)].
Product Categories/Family for anti-CTTN antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CTTN cttn (Catalog #AAA211940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Cortactin Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cortactin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CTTN cttn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cortactin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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