IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of CREB3L2 using anti-CREB3L2 antibody (AAA128201).CREB3L2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CREB3L2 Antibody (AAA128201) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
Rabbit anti-Human CREB3L2 Polyclonal Antibody | anti-CREB3L2 antibody
Anti-CREB3L2 Antibody Picoband
Gene Names
CREB3L2; BBF2H7
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
CREB3L2, Antibody; Anti-CREB3L2 Antibody Picoband; Cyclic AMP-responsive element-binding protein 3-like protein 2; cAMP responsive element binding protein 3 like 2; CREB3L2; BBF2H7; cAMP-responsive element-binding protein 3-like protein 2; BBF2 human homolog on chromosome 7 [Cleaved into: Processed cyclic AMP-responsive element-binding protein 3-like protein 2]; anti-CREB3L2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-CREB3L2 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CREB3L2, which shares 95.7% and 100% amino acid (aa) sequence identity with mouse and rat CREB3L2, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Cross Reactivity
No cross-reactivity with other proteins
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of CREB3L2 using anti-CREB3L2 antibody (AAA128201).CREB3L2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CREB3L2 Antibody (AAA128201) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of CREB3L2 using anti-CREB3L2 antibody (AAA128201).CREB3L2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CREB3L2 Antibody (AAA128201) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
WB (Western Blot)
(Figure 1. Western blot analysis of CREB3L2 using anti-CREB3L2 antibody (AAA128201).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEL whole cell lysates,Lane 2: human HUVEC whole cell lysates,Lane 3: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB3L2 antigen affinity purified polyclonal antibody (AAA128201) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CREB3L2 at approximately 60 kDa. The expected band size for CREB3L2 is at 57 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of CREB3L2 using anti-CREB3L2 antibody (AAA128201).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEL whole cell lysates,Lane 2: human HUVEC whole cell lysates,Lane 3: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB3L2 antigen affinity purified polyclonal antibody (AAA128201) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CREB3L2 at approximately 60 kDa. The expected band size for CREB3L2 is at 57 kDa.)
Related Product Information for anti-CREB3L2 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-CREB3L2 antibody
References
1. Bejarano, P. A., Padhya, T. A., Smith, R., Blough, R., Devitt, J. J., Gluckman, J. L. Hyalinizing spindle cell tumor with giant rosettes--a soft tissue tumor with mesenchymal and neuroendocrine features: an immunohistochemical, ultrastructural, and cytogenetic analysis. Arch. Path. Lab. Med. 124: 1179-1184, 2000.2. Panagopoulos, I., Storlazzi, C. T., Fletcher, C. D. M., Fletcher, J. A., Nascimento, A., Domanski, H. A., Wejde, J., Brosjo, O., Rydholm, A., Isaksson, M., Mandahl, N., Mertens, F. The chimeric FUS/CREB3L2 gene is specific for low-grade fibromyxoid sarcoma. Genes Chromosomes Cancer 40: 218-228, 2004.3. Reid, R., de Silva, M. V. C., Paterson, L., Ryan, E., Fisher, C. Low-grade fibromyxoid sarcoma and hyalinizing spindle cell tumor with giant rosettes share a common t(7;16)(q34;p11) translocation. Am. J. Surg. Path. 27: 1229-1236, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,415 Da
NCBI Official Full Name
cyclic AMP-responsive element-binding protein 3-like protein 2 isoform 2
NCBI Official Synonym Full Names
cAMP responsive element binding protein 3-like 2
NCBI Official Symbol
CREB3L2
NCBI Official Synonym Symbols
BBF2H7
NCBI Protein Information
cyclic AMP-responsive element-binding protein 3-like protein 2; TCAG_1951439; FUS/BBF2H7 protein; B-ZIB transcription factor; basic transcription factor 2; BBF2 human homolog on chromosome 7; cAMP-responsive element-binding protein 3-like protein 2
UniProt Protein Name
Cyclic AMP-responsive element-binding protein 3-like protein 2
UniProt Gene Name
CREB3L2
UniProt Synonym Gene Names
BBF2H7; cAMP-responsive element-binding protein 3-like protein 2
UniProt Entry Name
CR3L2_HUMAN
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Product Notes
The CREB3L2 creb3l2 (Catalog #AAA128201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CREB3L2 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CREB3L2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CREB3L2 creb3l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CREB3L2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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