ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2165 or CX2255).)
Rabbit CRMP2 Polyclonal Antibody | anti-CRMP2 antibody
CRMP2 (Thr-555), phospho-specific Antibody
Gene Names
DPYSL2; DRP2; N2A3; CRMP2; DRP-2; ULIP2; CRMP-2; DHPRP2; ULIP-2
Reactivity
Human, Rat, Mouse
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
CRMP2, Antibody; CRMP2 (Thr-555), phospho-specific Antibody; CRMP2 (Thr-555), phospho-specific; CRMP2 T555; anti-CRMP2 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to unphosphorylated CRMP2 (Thr-555) before affinity purification using phospho-CRMP2 (Thr-555) peptide (without carrier). The antibody detects a 70kDa* protein corresponding to CRMP2 on immunoblots of rat PC12 or mouse C2C12 cells treated with calyculin A. This reactivity can be specifically blocked using phospho-CRMP2 (Thr-555) peptide (CX2255).
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CRMP2 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Phospho-CRMP2 (Thr-555) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-555 in human CRMP2. This sequence is conserved in rat and mouse CRMP2 and the phospho-site is not conserved in other CRMP family members.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2165 or CX2255).)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2165 or CX2255).)
WB (Western Blot)
(Western blot of rat PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).)
WB (Western Blot)
(Western blot of rat PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).)
Related Product Information for anti-CRMP2 antibody
CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518, Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.
References
Cole, A.R. et al. (2006) J. Biol. Chem. 281(24):16591.
Mimura, F. et al. (2006) J. Biol. Chem. 281(23):15970.
Arimura, N. et al. (2005) Mol. Cell. Biol. 25(22):9973.
Uchida, Y. et al. (2005) Genes to Cells 10:165.
Arimura, N. et al. (2000) J. Biol. Chem. 275(31):23973
Mimura, F. et al. (2006) J. Biol. Chem. 281(23):15970.
Arimura, N. et al. (2005) Mol. Cell. Biol. 25(22):9973.
Uchida, Y. et al. (2005) Genes to Cells 10:165.
Arimura, N. et al. (2000) J. Biol. Chem. 275(31):23973
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,294 Da
NCBI Official Full Name
dihydropyrimidinase-related protein 2 isoform 1
NCBI Official Synonym Full Names
dihydropyrimidinase-like 2
NCBI Official Symbol
DPYSL2
NCBI Official Synonym Symbols
DRP2; N2A3; CRMP2; DRP-2; ULIP2; CRMP-2; DHPRP2; ULIP-2
NCBI Protein Information
dihydropyrimidinase-related protein 2; unc-33-like phosphoprotein 2; collapsin response mediator protein hCRMP-2
UniProt Protein Name
Dihydropyrimidinase-related protein 2
UniProt Gene Name
DPYSL2
UniProt Synonym Gene Names
CRMP2; ULIP2; DRP-2; CRMP-2; ULIP-2
UniProt Entry Name
DPYL2_HUMAN
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Product Notes
The CRMP2 dpysl2 (Catalog #AAA71605) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CRMP2 (Thr-555), phospho-specific Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CRMP2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CRMP2 dpysl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRMP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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