WB (Western Blot)
(Detection of human CRSP2-DRIP150 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, MCF-7, LNCaP, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CRSP2-DRIP150 antibody (AAA211291 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human CRSP2/DRIP150 Polyclonal Antibody | anti-CRSP2/DRIP150 antibody
Rabbit anti-CRSP2/DRIP150 Antibody, Affinity Purified
Gene Names
MED14; CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CRSP2/DRIP150, Antibody; Rabbit anti-CRSP2/DRIP150 Antibody, Affinity Purified; TRAP170; Mediator complex subunit 14; mediator of RNA polymerase II transcription subunit 14; RGR1; RGR1 homolog; Transcriptional coactivator CRSP150; vitamin D receptor-interacting protein complex component DRIP150; vitamin D3 receptor-interacting protein complex 150 kDa component; CXorf4; human homolog of yeast RGR1; thyroid hormone receptor-associated protein complex component TRAP170; ARC150; hRGR1; EXLM1; activator-recruited cofactor 150 kDa component; Cofactor required for Sp1 transcriptional activation subunit 2; CRSP complex subunit 2; CRSP150; CRSP2; CSRP; DRIP150; Mediator of RNA polymerase II transcription subunit 14; mediator complex subunit 14; anti-CRSP2/DRIP150 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CRSP2/DRIP150 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CRSP2-DRIP150 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, MCF-7, LNCaP, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CRSP2-DRIP150 antibody (AAA211291 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human CRSP2-DRIP150 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, MCF-7, LNCaP, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CRSP2-DRIP150 antibody (AAA211291 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CRSP2-DRIP150 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CRSP2-DRIP150 antibody (AAA211291 lot 2) used for IP at 6 ug per reaction. CRSP2-DRIP150 was also immunoprecipitated by a previous lot of this antibody (AAA211291 lot 1) and a second antibody against a different epitope of CRSP2-DRIP150 (BL1751). For blotting immunoprecipitated CRSP2-DRIP150, AAA211291 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CRSP2-DRIP150 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CRSP2-DRIP150 antibody (AAA211291 lot 2) used for IP at 6 ug per reaction. CRSP2-DRIP150 was also immunoprecipitated by a previous lot of this antibody (AAA211291 lot 1) and a second antibody against a different epitope of CRSP2-DRIP150 (BL1751). For blotting immunoprecipitated CRSP2-DRIP150, AAA211291 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-CRSP2/DRIP150 antibody
CRSP2 is a subunit of the CRSP (cofactor required for SP1 activation) complex required for efficient transcriptional activation by the SP1 transcription factor. It has also been identified as a component of the mediator complex that bridges nuclear hormone receptors to RNA polymerase II.
Product Categories/Family for anti-CRSP2/DRIP150 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
160,607 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 14
NCBI Official Synonym Full Names
mediator complex subunit 14
NCBI Official Symbol
MED14
NCBI Official Synonym Symbols
CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 14; hRGR1; ARC150; RGR1 homolog; CRSP complex subunit 2; human homolog of yeast RGR1; transcriptional co-activator CRSP150; activator-recruited cofactor 150 kDa component; vitamin D receptor-interacting
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 14
UniProt Gene Name
MED14
UniProt Synonym Gene Names
ARC150; CRSP2; CXorf4; DRIP150; EXLM1; RGR1; TRAP170; ARC150; CRSP complex subunit 2; hRGR1; Trap170; DRIP150
UniProt Entry Name
MED14_HUMAN
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Product Notes
The CRSP2/DRIP150 med14 (Catalog #AAA211291) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CRSP2/DRIP150 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CRSP2/DRIP150 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CRSP2/DRIP150 med14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRSP2/DRIP150, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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