Rabbit anti-Human CSNK2A2 Polyclonal Antibody | anti-CSNK2A2 antibody

Anti-CSNK2A2 Antibody, Rabbit Polyclonal

Cat. Num. AAA256962

• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: CSNK2A2, Antibody; Anti-CSNK2A2 Antibody, Rabbit Polyclonal; CSNK2A2 Antibody, Rabbit PAb, Antigen Affinity Purified; casein kinase 2, alpha prime polypeptide; Anti-CK2A2 Antibody; Anti-CSNK2A1 Antibody; anti-CSNK2A2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CSNK2A2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-CSNK2A2 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
• Immunogen: E Coli-derived Human CSNK2A2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human CSNK2A2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence staining of CSNK2A2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CSNK2A2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human CSNK2A2 in human ovarian cancer with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining of human CSNK2A2 in human testis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-CSNK2A2 antibody

Casein kinase II subunit alpha', also known as CSNK2A2 and CK2A2, is a member of the protein kinase superfamily, Ser/Thr protein kinase family and CK2 subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. CSNK2A2 is a tetramer composed of an alpha chain, an alpha' and two beta chains. It is also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Protein kinase casein kinase II (Ck2) is a cyclic-AMP and calcium-independent serine-threonine kinase that is composed of two catalytic subunits (alpha and alpha') and two regulatory beta-subunits. Ck2 is not a casein kinase in vivo, but over 1 substrates are known. The highly conserved amino acid sequences of its subunits and their broad expression suggest that Ck2 may have a fundamental role in cell function. Ck2 has been implicated in DNA replication, regulation of basal and inducible transcription, translation and control of metabolism. The Ck2alpha and Ck2alpha' isoforms (products of the genes Csnk2a1 and Csnk2a2, respectively) are highly homologous, the reason for their redundancy and evolutionary conservation is unknown. CSNK2A2 may be a candidate gene for these inherited syndromes.

References

Xu X. et al., 1999, Nat Genet. 23 (1):118-21. Aasland M. et al., 2000, Anim Genet. 31 (2):131-4. Escalier D. et al., 2003, Mol Reprod Dev. 66 (2):190-201. Ackermann K. et al., 2005, Mol Cell Biochem. 274 (1-2):91-101.