IF (Immunofluorescence)
(Fluorescent confocal image of SK-BR-3 cell stained with CTBP1 Antibody (C-term). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.)
Rabbit CTBP1 Polyclonal Antibody | anti-CTBP1 antibody
CTBP1 Antibody (C-term)
Gene Names
CTBP1; BARS
Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CTBP1, Antibody; CTBP1 Antibody (C-term); C-terminal-binding protein 1; CtBP1; 111-; CTBP1; CTBP; anti-CTBP1 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CTBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human CTBP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
413-440
Sequence Length
429
Applicable Applications for anti-CTBP1 antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of SK-BR-3 cell stained with CTBP1 Antibody (C-term). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of SK-BR-3 cell stained with CTBP1 Antibody (C-term). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with CTBP1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with CTBP1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.)
WB (Western Blot)
(CTBP1 Antibody (C-term) western blot analysis in Hela,HL-60,Jurkat,Raji cell line and mouse brain,rat brain tissue lysates (35ug/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow).)
WB (Western Blot)
(CTBP1 Antibody (C-term) western blot analysis in Hela,HL-60,Jurkat,Raji cell line and mouse brain,rat brain tissue lysates (35ug/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow).)
Related Product Information for anti-CTBP1 antibody
This gene encodes a protein that binds to the C-terminus
of adenovirus E1A proteins. This phosphoprotein is a
transcriptional repressor and may play a role during cellular
proliferation. This protein and the product of a second closely
related gene, CTBP2, can dimerize. Both proteins can also interact
with a polycomb group protein complex which participates in
regulation of gene expression during development. Alternative
splicing of transcripts from this gene results in multiple
transcript variants.
of adenovirus E1A proteins. This phosphoprotein is a
transcriptional repressor and may play a role during cellular
proliferation. This protein and the product of a second closely
related gene, CTBP2, can dimerize. Both proteins can also interact
with a polycomb group protein complex which participates in
regulation of gene expression during development. Alternative
splicing of transcripts from this gene results in multiple
transcript variants.
Product Categories/Family for anti-CTBP1 antibody
References
Roukens, M.G., et al. Nat. Cell Biol. 12(10):933-942(2010)
Choi, H.J., et al. Biochem. Biophys. Res. Commun. 400(3):396-402(2010)
Merrill, J.C., et al. J. Mol. Biol. 398(5):657-671(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Choi, H.J., et al. Biochem. Biophys. Res. Commun. 400(3):396-402(2010)
Merrill, J.C., et al. J. Mol. Biol. 398(5):657-671(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47535
NCBI Official Full Name
C-terminal-binding protein 1 isoform 2
NCBI Official Synonym Full Names
C-terminal binding protein 1
NCBI Official Symbol
CTBP1
NCBI Official Synonym Symbols
BARS
NCBI Protein Information
C-terminal-binding protein 1
UniProt Protein Name
C-terminal-binding protein 1
UniProt Gene Name
CTBP1
UniProt Synonym Gene Names
CTBP; CtBP1
UniProt Entry Name
CTBP1_HUMAN
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Product Notes
The CTBP1 ctbp1 (Catalog #AAA283660) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 413-440. The CTBP1 Antibody (C-term) reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's CTBP1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the CTBP1 ctbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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