IP (Immunoprecipitation)
(Detection of human Cul1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cul1 antibody AAA212157 used for IP at 6 ug/mg lysate. Cul1 was also immunoprecipitated by rabbit anti-Cul1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated Cul1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human Cul1 Polyclonal Antibody | anti-Cul1 antibody
Rabbit anti-Cul1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
Cul1, Antibody; Rabbit anti-Cul1 Antibody, Affinity Purified; CUL-1; cullin-1; Cullin-1; cullin 1; anti-Cul1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Cul1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse, Orangutan
Immunogen
Between 275 and 325
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human Cul1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cul1 antibody AAA212157 used for IP at 6 ug/mg lysate. Cul1 was also immunoprecipitated by rabbit anti-Cul1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated Cul1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Cul1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cul1 antibody AAA212157 used for IP at 6 ug/mg lysate. Cul1 was also immunoprecipitated by rabbit anti-Cul1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated Cul1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human Cul1 by immunohistochemistry. Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti-Cul1 (Cat. No. AAA212157 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Cul1 by immunohistochemistry. Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti-Cul1 (Cat. No. AAA212157 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Cul1 antibody
Cullin 1 (Cul1) is the core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, Cul1 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul1 may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13616].
Product Categories/Family for anti-Cul1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,679 Da
NCBI Official Full Name
cullin-1
NCBI Official Synonym Full Names
cullin 1
NCBI Official Symbol
CUL1
NCBI Protein Information
cullin-1; CUL-1
UniProt Protein Name
Cullin-1
UniProt Gene Name
CUL1
UniProt Synonym Gene Names
CUL-1
UniProt Entry Name
CUL1_HUMAN
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Product Notes
The Cul1 cul1 (Catalog #AAA212157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Cul1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cul1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the Cul1 cul1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cul1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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