Rabbit CUL1 Polyclonal Antibody | anti-CUL1 antibody

CUL1 Antibody

Cat. Num. AAA303633

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: CUL1, Antibody; CUL1 Antibody; MGC149834; MGC149835; anti-CUL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total CUL1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 776

• Applicable Applications for anti-CUL1 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human CUL1.
• Target Name: CUL1
• Preparation and Storage: Store at -20 degree C

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer using CUL1 antibody at dilution of 1:200 (200x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using CUL1 antibody.)

Related Product Information for anti-CUL1 antibody

Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggest that activated E2 associates transiently with E3, and that the dissociation is a critical step for ubiqitination (5). Cullin homolog 1 (CUL1), the mammalian homolog of Cdc53 from yeast, is a molecular scaffold of the SCF (Skp1/CUL1/F-box) E3 ubiquitin ligase protein complex. Thus, CUL1 and its family members function in ubiquitin dependent proteolysis (6). In particular, CUL1 has been shown to mediate ubiquitin dependent degradation of p21 Waf1/Cip1, cyclin D and IkappaB-alpha (7,8).

Product Categories/Family for anti-CUL1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 32307161
• NCBI GeneID: 8454
• NCBI Accession #: NP_003583.2
• NCBI GenBank Nucleotide #: NM_003592.2
• UniProt Accession #: Q13616; O60719; Q08AL6; Q8IYW1; D3DWG3
• Molecular Weight: 89,679 Da
• NCBI Official Full Name: cullin-1
• NCBI Official Synonym Full Names: cullin 1
• NCBI Official Symbol: CUL1
• NCBI Protein Information: cullin-1
• UniProt Protein Name: Cullin-1
• UniProt Gene Name: CUL1
• UniProt Synonym Gene Names: CUL-1
• UniProt Entry Name: CUL1_HUMAN