WB (Western Blot)
(Anti-Cullin 2 antibody, AAA45405, Western blottingLane 1: A431 Cell LysateLane 2: SMMC Cell LysateLane 3: HELA Cell LysateLane 4: COLO320 Cell Lysate)
Rabbit Cullin 2 Polyclonal Antibody | anti-CUL2 antibody
Anti-Cullin 2 antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Cullin 2, Antibody; Anti-Cullin 2 antibody; Cullin-2; cullin 2; Cul2 antibody; CUL 2 antibody; CUL-2 antibody; CUL2 antibody; CUL2_HUMAN antibody; cullin 2 antibody; cullin homolog 2 antibody; Cullin-2 antibody; MGC131970 antibody; anti-CUL2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
745
Applicable Applications for anti-CUL2 antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Cullin 2 (264-280aa HECQQRMVADHLQFLHA), different from the related mouse and rat sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-Cullin 2 antibody, AAA45405, Western blottingLane 1: A431 Cell LysateLane 2: SMMC Cell LysateLane 3: HELA Cell LysateLane 4: COLO320 Cell Lysate)
WB (Western Blot)
(Anti-Cullin 2 antibody, AAA45405, Western blottingLane 1: A431 Cell LysateLane 2: SMMC Cell LysateLane 3: HELA Cell LysateLane 4: COLO320 Cell Lysate)
WB (Western Blot)
(Anti-Cullin 2 antibody, AAA45405, Western blottingWB: Rat Brain Tissue Lysate)
WB (Western Blot)
(Anti-Cullin 2 antibody, AAA45405, Western blottingWB: Rat Brain Tissue Lysate)
WB (Western Blot)
(Anti-Cullin 2 antibody, AAA45405, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant human Cul2, 46.5KD(162aa tag+L150-Y394)Lane 1: Recombinant Human Cul2 Protein 10ngLane 2: Recombinant Human Cul2 Protein 5ngLane 3: Recombinant Human Cul2 Protein 2.5ng)
WB (Western Blot)
(Anti-Cullin 2 antibody, AAA45405, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant human Cul2, 46.5KD(162aa tag+L150-Y394)Lane 1: Recombinant Human Cul2 Protein 10ngLane 2: Recombinant Human Cul2 Protein 5ngLane 3: Recombinant Human Cul2 Protein 2.5ng)
Related Product Information for anti-CUL2 antibody
Description: Rabbit IgG polyclonal antibody for Cullin-2(CUL2) detection. Tested with WB in Human, Mouse, Rat.
Background: Cullin 2(CUL2) is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, they showed that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, Clifford et al.(1999)mapped the CUL2 gene to 10p11.2-p11.1, a region reported to show loss of heterozygosity(LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. Pause et al.(1997)suggested that CUL2 is a candidate tumor suppressor gene, as has been proposed for CUL1(603134). Lonergan et al.(1998) demonstrated that formation of the VBC-CUL2 complexes is linked to the regulation of hypoxia-inducible mRNAs by VHL. Cul2 was one of several proteins required for degradation of a class of RNA-binding germline proteins in somatic cells of the early blastomere.
Background: Cullin 2(CUL2) is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, they showed that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, Clifford et al.(1999)mapped the CUL2 gene to 10p11.2-p11.1, a region reported to show loss of heterozygosity(LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. Pause et al.(1997)suggested that CUL2 is a candidate tumor suppressor gene, as has been proposed for CUL1(603134). Lonergan et al.(1998) demonstrated that formation of the VBC-CUL2 complexes is linked to the regulation of hypoxia-inducible mRNAs by VHL. Cul2 was one of several proteins required for degradation of a class of RNA-binding germline proteins in somatic cells of the early blastomere.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
89,492 Da
NCBI Official Full Name
Cullin-2
NCBI Official Synonym Full Names
cullin 2
NCBI Official Symbol
CUL2
NCBI Protein Information
cullin-2; CUL-2
UniProt Protein Name
Cullin-2
UniProt Gene Name
CUL2
UniProt Synonym Gene Names
CUL-2
UniProt Entry Name
CUL2_HUMAN
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Product Notes
The CUL2 cul2 (Catalog #AAA45405) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cullin 2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cullin 2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CUL2 cul2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cullin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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