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WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 antiobdy. The lane on the left is treated with systhesized peptide.)
Rabbit anti-Human Cullin 2 Polyclonal Antibody | anti-CUL2 antibody
Cullin 2 Antibody
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Cullin 2, Antibody; Cullin 2 Antibody; Cullin-2; CUL-2; anti-CUL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total Cullin 2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
745
Applicable Applications for anti-CUL2 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from human Cullin 2.
Target Name
Cullin 2
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 antiobdy. The lane on the left is treated with systhesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 antiobdy. The lane on the left is treated with systhesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from LOVO cells, using Cullin 2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from LOVO cells, using Cullin 2 antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody.)
Related Product Information for anti-CUL2 antibody
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 By similarity. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).Seiji Shiraishi, J. Biol. Chem., Mar 2007; 282: 9017 - 9028.Isabelle Groulx, J. Biol. Chem., Mar 2000; 275: 8991.Dimitris Liakopoulos, PNAS, May 1999; 96: 5510.Kyungwon Huh, AACR Meeting Abstracts, Apr 2006; 2006: 1326.
Product Categories/Family for anti-CUL2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,492 Da
NCBI Official Full Name
cullin-2 isoform c
NCBI Official Synonym Full Names
cullin 2
NCBI Official Symbol
CUL2
NCBI Protein Information
cullin-2
UniProt Protein Name
Cullin-2
UniProt Gene Name
CUL2
UniProt Synonym Gene Names
CUL-2
UniProt Entry Name
CUL2_HUMAN
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Product Notes
The CUL2 cul2 (Catalog #AAA306871) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cullin 2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cullin 2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CUL2 cul2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cullin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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