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FCM/FACS (Flow Cytometry)
(CYP21A2 Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CYP21A2 Polyclonal Antibody | anti-CYP21A2 antibody
Anti-CYP21A2 Antibody (aa224-253) IHC-plus
Gene Names
CYP21A2; CAH1; CPS1; CA21H; CYP21; CYP21B; P450c21B
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Ammonium sulfate precipitation
Synonyms
CYP21A2, Antibody; Anti-CYP21A2 Antibody (aa224-253) IHC-plus; Rabbit Polyclonal to Human CYP21A2; Human CYP21A2; 21-OHase; CAH1; CYP21B; Cytochrome P450-C21; Cytochrome P450 21; Cytochrome P450 XXI; p450c21B; CA21H; CYP21; Cytochrome P-450c21; Cytochrome P450-C21B; Steroid 21-hydroxylase; Steroid 21-monooxygenase; anti-CYP21A2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human CYP21A2
Purity/Purification
Ammonium sulfate precipitation
Form/Format
PBS, 0.09% sodium azide
Sequence Length
495
Applicable Applications for anti-CYP21A2 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
CYP21A2 antibody was raised against kLH-conjugated synthetic peptide from internal region of human CYP21A2.
Immunogen Description
KLH-conjugated synthetic peptide from internal region of human CYP21A2.
Immunogen Type
Synthetic peptide
Antigen Modification
aa224-253
Target Species
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
For long term storage store at -20 degree C.
FCM/FACS (Flow Cytometry)
(CYP21A2 Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CYP21A2 Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot of CYP21A2 Antibody in MDA-MB435 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.)
WB (Western Blot)
(Western blot of CYP21A2 Antibody in MDA-MB435 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.)
IF (Immunofluorescence)
(Confocal immunofluorescence of CYP21A2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescence of CYP21A2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemistry)
(Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
Related Product Information for anti-CYP21A2 antibody
CYP21A2 Antibody, 21-OHase Antibody, CAH1 Antibody, CYP21B Antibody, Cytochrome P450-C21 Antibody, Cytochrome P450 21 Antibody, Cytochrome P450 XXI Antibody, p450c21B Antibody, CA21H Antibody, CYP21 Antibody, Cytochrome P-450c21 Antibody, Cytochrome P450-C21B Antibody, Steroid 21-hydroxylase Antibody, Steroid 21-monooxygenase Antibody Description: CYP21A2 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,597 Da
NCBI Official Full Name
steroid 21-hydroxylase isoform a
NCBI Official Synonym Full Names
cytochrome P450 family 21 subfamily A member 2
NCBI Official Symbol
CYP21A2
NCBI Official Synonym Symbols
CAH1; CPS1; CA21H; CYP21; CYP21B; P450c21B
NCBI Protein Information
steroid 21-hydroxylase
UniProt Protein Name
Steroid 21-hydroxylase
UniProt Gene Name
CYP21A2
UniProt Synonym Gene Names
CYP21; CYP21B
UniProt Entry Name
CP21A_HUMAN
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Product Notes
The CYP21A2 cyp21a2 (Catalog #AAA52673) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CYP21A2 Antibody (aa224-253) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYP21A2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CYP21A2 cyp21a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYP21A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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