IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Immunofluorescent analysis of Cytochrome P450 11A1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Rabbit Cytochrome P450 11A1 Polyclonal Antibody | anti-CYP11A1 antibody
Anti-Cytochrome P450 11A1 Antibody
Gene Names
CYP11A1; CYP11A; CYPXIA1; P450SCC
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunofluorescence, Western Blot
Synonyms
Cytochrome P450 11A1, Antibody; Anti-Cytochrome P450 11A1 Antibody; CYP11A; Cholesterol side-chain cleavage enzyme mitochondrial; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450 (scc); anti-CYP11A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Applicable Applications for anti-CYP11A1 antibody
IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Notes
ECO:0000303|PubMed:21636783, ECO:0000312|HGNC: HGNC:2590
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Immunofluorescent analysis of Cytochrome P450 11A1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Immunofluorescent analysis of Cytochrome P450 11A1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Western blot analysis of Cytochrome P450 11A1 expression in HEK293T (A), Hela (B), H460 (C), mouse lung (D) whole cell lysates.)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Western blot analysis of Cytochrome P450 11A1 expression in HEK293T (A), Hela (B), H460 (C), mouse lung (D) whole cell lysates.)
Related Product Information for anti-CYP11A1 antibody
Rabbit polyclonal antibody to Cytochrome P450 11A1
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytochrome P450 11A1. The exact sequence is proprietary.
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytochrome P450 11A1. The exact sequence is proprietary.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,102 Da
NCBI Official Full Name
cholesterol side-chain cleavage enzyme, mitochondrial isoform a
NCBI Official Synonym Full Names
cytochrome P450, family 11, subfamily A, polypeptide 1
NCBI Official Symbol
CYP11A1
NCBI Official Synonym Symbols
CYP11A; CYPXIA1; P450SCC
NCBI Protein Information
cholesterol side-chain cleavage enzyme, mitochondrial; steroid 20-22-lyase; cytochrome P450 11A1; cytochrome P450(scc); cytochrome P450C11A1; cholesterol 20-22 desmolase; cholesterol monooxygenase (side-chain cleaving); cytochrome P450, subfamily XIA (cho
UniProt Protein Name
Cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Gene Name
CYP11A1
UniProt Synonym Gene Names
CYP11A
UniProt Entry Name
CP11A_HUMAN
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Product Notes
The CYP11A1 cyp11a1 (Catalog #AAA292271) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytochrome P450 11A1 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 11A1 can be used in a range of immunoassay formats including, but not limited to, IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CYP11A1 cyp11a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 11A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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