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product-image-AAA40588_WB10.gif WB (Western Blot) (Western blot analysis of DAF1 expression in HeLa cell lysate with DAF1 antibody at (A) 1 and (B) 2 μg/ml.)

Rabbit anti-Human DAF Polyclonal Antibody | anti-CD55 antibody

DAF Antibody

Gene Names
CD55; CR; TC; DAF; CROM
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
DAF antibody is affinity chromatography purified via peptide column.
Synonyms
DAF, Antibody; DAF Antibody; Decay-accelerating factor; CD55 molecule decay accelerating factor for complement (Cromer blood group); CD55; CR; TC; CROM; CD55 molecule, decay accelerating factor for complement (Cromer blood group); anti-CD55 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.
Purity/Purification
DAF antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
440
Applicable Applications for anti-CD55 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF.
Buffer
DAF antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
DAF antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

WB (Western Blot)

(Western blot analysis of DAF1 expression in HeLa cell lysate with DAF1 antibody at (A) 1 and (B) 2 μg/ml.)

product-image-AAA40588_WB10.gif WB (Western Blot) (Western blot analysis of DAF1 expression in HeLa cell lysate with DAF1 antibody at (A) 1 and (B) 2 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.)

product-image-AAA40588_IF11.jpg IF (Immunofluorescence) (Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.)

product-image-AAA40588_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.)

WB (Western Blot)

(Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.)

product-image-AAA40588_WB15.gif WB (Western Blot) (Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.)
Related Product Information for anti-CD55 antibody
The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 48 kDa

Observed: 49 kDa
NCBI Official Full Name
complement decay-accelerating factor isoform 2
NCBI Official Synonym Full Names
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
NCBI Official Symbol
CD55
NCBI Official Synonym Symbols
CR; TC; DAF; CROM
NCBI Protein Information
complement decay-accelerating factor; CD55 antigen
UniProt Protein Name
Complement decay-accelerating factor
UniProt Gene Name
CD55
UniProt Synonym Gene Names
CR; DAF
UniProt Entry Name
DAF_HUMAN

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Product Notes

The CD55 cd55 (Catalog #AAA40588) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAF Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DAF can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CD55 cd55 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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