WB (Western Blot)
(All lanes : Anti-(DANRE) atf4 Antibody(C-term) at 1:1000 dilutionLane 1: Zebrafish lysateLane 2: Zebrafish muscle lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST)
Rabbit anti-Zebrafish DANRE atf4 Polyclonal Antibody | anti-atf4 antibody
DANRE atf4 Antibody (C-term)
Gene Names
atf4a; atf4; atf4b1; zgc:85951; wu:fj01c08; wu:fk30b04
Reactivity
Zebrafish
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DANRE atf4, Antibody; DANRE atf4 Antibody (C-term); Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; atf4 {ECO:0000312|ZFIN:ZDB-GENE-040426-2340}; atf4b1; anti-atf4 antibody
Host
Rabbit
Reactivity
Zebrafish
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DANRE atf4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-314 amino acids from the C-terminal region of DANRE atf4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.79 (varies by lot)
Sequence Positions
288-314
Sequence Length
339
Applicable Applications for anti-atf4 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Function
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. May play a role in the regulation of the circadian clock (By similarity).
Cellular Location
Cytoplasm. Cell membrane. Nucleus {ECO:0000250|UniProtKB:P18848, ECO:0000250|UniProtKB:Q9ES19, ECO:0000255|PROSITE-ProRule:PRU00978}
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-(DANRE) atf4 Antibody(C-term) at 1:1000 dilutionLane 1: Zebrafish lysateLane 2: Zebrafish muscle lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST)
WB (Western Blot)
(All lanes : Anti-(DANRE) atf4 Antibody(C-term) at 1:1000 dilutionLane 1: Zebrafish lysateLane 2: Zebrafish muscle lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST)
Related Product Information for anti-atf4 antibody
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters (By similarity).
Product Categories/Family for anti-atf4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36605
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-4
NCBI Official Synonym Full Names
activating transcription factor 4a
NCBI Official Symbol
atf4a
NCBI Official Synonym Symbols
atf4; atf4b1; zgc:85951; wu:fj01c08; wu:fk30b04
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-4
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-4
UniProt Gene Name
atf4
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-4
UniProt Entry Name
ATF4_DANRE
Similar Products
Product Notes
The atf4 atf4 (Catalog #AAA283964) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 288-314. The DANRE atf4 Antibody (C-term) reacts with Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's DANRE atf4 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the atf4 atf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DANRE atf4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.