Rabbit anti-Zebrafish DANRE shha Polyclonal Antibody | anti-shha antibody

DANRE shha Antibody (Center)

Cat. Num. AAA288398

• Gene Names: shha; shh; syu; vhh1; vhh-1; fc83d08; wu:fc83d08
• Reactivity: Zebrafish
• Applications: ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DANRE shha, Antibody; DANRE shha Antibody (Center); Sonic hedgehog protein A; SHHA; VHH-1; Sonic hedgehog protein A N-product; Sonic hedgehog protein A C-product; shha; shh; vhh1; anti-shha antibody

• Host: Rabbit
• Reactivity: Zebrafish
• Clonality: Polyclonal
• Specificity: This shha antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 151-162 amino acids from the Central region of DANRE shha.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 418

• Applicable Applications for anti-shha antibody: ELISA, WB (Western Blot)
• Function: [Sonic hedgehog protein]: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity).
• Cellular Location: [Sonic hedgehog protein]: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q15465}. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q15465}. Note=Co-localizes with HHAT in the ER and Golgi membrane. {ECO:0000250|UniProtKB:Q15465}
• Tissue Location: Expressed in the ventral midline of the neural tube and brain. Also found in the notochord and in developing fin bud. In the developing brain, expression occurs in domains that include a discrete region in the floor of the diencephalon
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Shha Antibody (Center) western blot analysis in zebrafish brain tissue lysate (35ug/lane). This demonstrates that the (DANRE) shha antibody detected zebrafish shha protein (arrow).)

Related Product Information for anti-shha antibody

Intercellular signal essential for a variety of patterning events during development. Signal produced by the notochord that induces somite patterning, dorso-ventral patterning of the brain and early patterning of the developing eyes. Displays floor plate-inducing activity. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO (By similarity).

Product Categories/Family for anti-shha antibody

Crown Antibodies

References

Roelink H.,et al.Cell 76:761-775(1994).
Ekker S.C.,et al.Curr. Biol. 5:944-955(1995).
Fietz M.J.,et al.Development Suppl. 120:43-51(1994).
Muller F.,et al.Development 126:2103-2116(1999).
Zardoya R.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:13036-13041(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 18859357
• NCBI GeneID: 30269
• NCBI Accession #: NP_571138.1
• NCBI GenBank Nucleotide #: NM_131063.3
• UniProt Accession #: Q92008; O13170; O13171; O13208; O13209; O13245; O13246
• Molecular Weight: 46403
• NCBI Official Full Name: sonic hedgehog protein A
• NCBI Official Synonym Full Names: sonic hedgehog a
• NCBI Official Symbol: shha
• NCBI Official Synonym Symbols: shh; syu; vhh1; vhh-1; fc83d08; wu:fc83d08
• NCBI Protein Information: sonic hedgehog protein A
• UniProt Protein Name: Sonic hedgehog protein A
• UniProt Gene Name: shha
• UniProt Synonym Gene Names: shh; vhh1; SHHA
• UniProt Entry Name: SHH_DANRE