Goat DAP3 Polyclonal Antibody | anti-DAP3 antibody

Goat anti-DAP3 Antibody

Cat. Num. AAA61123

• Gene Names: DAP3; DAP-3; MRPS29; MRP-S29; bMRP-10
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: Immunocytochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: DAP3, Antibody; Goat anti-DAP3 Antibody; DAP3; death associated protein 3; DAP-3; DKFZp686G12159; MGC126058; MGC126059; MRP-S29; MRPS29; bMRP-10; death-associated protein 3; mitochondrial 28S ribosomal protein S29; DAP3 antibody; death associated protein 3 antibody; DAP-3 antibody; DKFZp686G12159 antibody; MGC126058 antibody; MGC126059 antibody; MRP-S29 antibody; MRPS29 antibody; bMRP-10 antibody; death-associated protein 3 antibody; mitochondrial 28S ribosomal protein S29 antibody; anti-DAP3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: NPSLLERHCAYL
• Sequence Length: 398

• Applicable Applications for anti-DAP3 antibody: ICC (Immunocytochemistry), WB (Western Blot), ELISA (Peptide ELISA)
• Immunogen: Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1; NP_001186779.1; NP_001186780.1.
• Epitope: C Terminus
• Note: This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

IF (Immunofluorescence)

((0.5ug/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain.)

WB (Western Blot)

(HEK293 overexpressing DAP3 (RC223182) and probed (mock transfection in first lane), tested by Origene.)

WB (Western Blot)

(Staining (3ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

WB (Western Blot)

((1ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 16905526
• NCBI GeneID: 7818
• NCBI Accession #: NP_387506.1; NP_001186779.1; NP_001186780.1
• NCBI GenBank Nucleotide #: NM_033657.2
• Molecular Weight: 41,673 Da
• NCBI Official Full Name: 28S ribosomal protein S29, mitochondrial isoform 1
• NCBI Official Synonym Full Names: death associated protein 3
• NCBI Official Symbol: DAP3
• NCBI Official Synonym Symbols: DAP-3; MRPS29; MRP-S29; bMRP-10
• NCBI Protein Information: 28S ribosomal protein S29, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S29, mitochondrial
• UniProt Gene Name: DAP3
• UniProt Synonym Gene Names: MRPS29; MRP-S29; S29mt; DAP-3
• UniProt Entry Name: RT29_HUMAN