anti-Human, Rat Daxx Polyclonal Antibody | anti-DAXX antibody

Anti-Daxx Antibody

Cat. Num. AAA46520

• Gene Names: DAXX; DAP6; EAP1; BING2
• Reactivity: Human, Rat
• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Daxx, Antibody; Anti-Daxx Antibody; Death domain-associated protein 6; BING 2; BING2; CENP-C binding protein; DAP 6; DAP6; Daxx; DAXX; DAXX_HUMAN; Death associated protein 6; Death domain associated protein 6; Death domain associated protein; EAP 1; EAP1; ETS1 associated protein 1; ETS1-associated protein 1; Fas binding protein; Fas death domain associated protein; Fas death domain-associated protein; hDaxx; MGC126245; MGC126246 antibody; death-domain associated protein; anti-DAXX antibody

• Reactivity: Human, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 740

• Applicable Applications for anti-DAXX antibody: ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived human Daxx recombinant protein (Position: K56-R345). Human Daxx shares 88.3% and 88.6% amino acid (aa) sequence identity with mouse and rat Daxx, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

ICC (Immunocytochemistry)

(Anti- DAXX Picoband antibody, AAA46520,ICCICC: A549 Cell)

ICC (Immunocytochemistry)

(Anti- DAXX Picoband antibody, AAA46520,ICCICC: SMMC-7721 Cell)

IHC (Immunohistochemisry)

(Anti- DAXX Picoband antibody, AAA46520,IHC(P)IHC(P): Human Intestinal Cancer Tissue)

IHC (Immunohiostchemistry)

(Anti- DAXX Picoband antibody, AAA46520,IHC(P)IHC(P): Rat Intestine Tissue)

WB (Western Blot)

(Anti- DAXX Picoband antibody, AAA46520, Western blottingAll lanes: Anti DAXX (AAA46520) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: A431 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: HUT Whole Cell Lysate at 40ugLane 5: HEPA Whole Cell Lysate at 40ugPredicted bind size: 81KDObserved bind size: 130KD)

Related Product Information for anti-DAXX antibody

Description: Rabbit IgG polyclonal antibody for Death domain-associated protein 6(DAXX) detection. Tested with WB, IHC-P, ICC in Human;Rat.

Background: DAXX (death-domain associated protein) also known as DAP6 (Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. Human DAXX encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses by Yang et al. (1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.

References

1. Chang, H. Y., Nishitoh, H., Yang, X., Ichijo, H., Baltimore, D. Activation of apoptosis signal-regulating kinase 1 (ASK1) by the adapter protein Daxx. Science 281: 1860-1863, 1998. 2. Kiriakidou, M., Driscoll, D. A., Lopez-Guisa, J. M., Strauss, J. F., III. Cloning and expression of primate Daxx cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region. DNA Cell Biol. 16: 1289-1298, 1997. 3. Yang, X., Khosravi-Far, R., Chang, H. Y., Baltimore, D. Daxx, a novel Fas-binding protein that activates JNK and apoptosis. Cell 89: 1067-1076, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 215422388
• NCBI GeneID: 1616
• NCBI Accession #: NP_001135441.1
• NCBI GenBank Nucleotide #: NM_001141969.1
• UniProt Accession #: Q9UER7; O14747; O15141; O15208; Q5STK9; Q9BWI3; B4E1I3; F5ANJ6; F5ANJ7; F5H082
• Molecular Weight: 75,563 Da
• NCBI Official Full Name: death domain-associated protein 6 isoform a
• NCBI Official Synonym Full Names: death-domain associated protein
• NCBI Official Symbol: DAXX
• NCBI Official Synonym Symbols: DAP6; EAP1; BING2
• NCBI Protein Information: death domain-associated protein 6
• UniProt Protein Name: Death domain-associated protein 6
• UniProt Gene Name: DAXX
• UniProt Synonym Gene Names: BING2; DAP6; hDaxx; EAP1
• UniProt Entry Name: DAXX_HUMAN