WB (Western Blot)
(Detection of human DBC1/p30 DBC by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DBC1/p30 DBC antibody AAA210948 (lot number AAA210948-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. DBC1/p30 DBC was also immunoprecipitated by a previous lot (AAA210948-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
Rabbit anti-Human, Mouse DBC1/p30 DBC Polyclonal Antibody | anti-DBC1/p30DBC antibody
Rabbit anti-DBC1/p30 DBC Antibody, Affinity Purified
Gene Names
KIAA1967; DBC1; DBC-1; NET35; p30DBC; p30 DBC
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DBC1/p30 DBC, Antibody; Rabbit anti-DBC1/p30 DBC Antibody, Affinity Purified; DBC-1; p30DBC; p30 DBC protein; p30 DBC; NET35; KIAA1967; Deleted in breast cancer gene 1 protein; DBIRD complex subunit KIAA1967; DBC1; DBC.1; cell division cycle and apoptosis regulator protein 2; cell cycle and apoptosis regulator protein 2; deleted in breast cancer 1; Cell cycle and apoptosis regulator protein 2; cell cycle and apoptosis regulator 2; anti-DBC1/p30DBC antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DBC1/p30DBC antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 873 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DBC1/p30 DBC by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DBC1/p30 DBC antibody AAA210948 (lot number AAA210948-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. DBC1/p30 DBC was also immunoprecipitated by a previous lot (AAA210948-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
WB (Western Blot)
(Detection of human DBC1/p30 DBC by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DBC1/p30 DBC antibody AAA210948 (lot number AAA210948-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. DBC1/p30 DBC was also immunoprecipitated by a previous lot (AAA210948-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
WB (Western Blot)
(Detection of mouse DBC1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-DBC1 antibody AAA210948 (lot AAA210948-2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse DBC1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-DBC1 antibody AAA210948 (lot AAA210948-2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human DBC1/p30 DBC by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DBC1/p30 DBC (Cat. No. AAA210948 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DBC1/p30 DBC by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DBC1/p30 DBC (Cat. No. AAA210948 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-DBC1/p30DBC antibody
The DBC1 (Deleted in Breast Cancer Gene 1 protein) gene is located in a region frequently deleted in breast cancers. In its uncleaved form, DBC1 is localized to the nucleus. Upon cleavage by caspase activity, DBC1 becomes localized to the cytoplasm and functions to promote apoptosis. DBC1 has also been found to bind directly to the estrogen receptor alpha (ERalpha) hormone binding domain to modulate ERalpha expression and mediate hormone-independent breast cancer cell survival.
Product Categories/Family for anti-DBC1/p30DBC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
102,902 Da
NCBI Official Full Name
DBIRD complex subunit KIAA1967
NCBI Official Synonym Full Names
KIAA1967
NCBI Official Symbol
KIAA1967
NCBI Official Synonym Symbols
DBC1; DBC-1; NET35; p30DBC; p30 DBC
NCBI Protein Information
DBIRD complex subunit KIAA1967; p30 DBC protein; deleted in breast cancer 1
UniProt Protein Name
DBIRD complex subunit KIAA1967
UniProt Gene Name
KIAA1967
UniProt Synonym Gene Names
DBC1; DBC-1; DBC.1
UniProt Entry Name
K1967_HUMAN
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Product Notes
The DBC1/p30DBC kiaa1967 (Catalog #AAA210948) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DBC1/p30 DBC Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DBC1/p30 DBC can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DBC1/p30DBC kiaa1967 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DBC1/p30 DBC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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