WB (Western Blot)
(Anti-DCUN1D1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:30 kDa)
Rabbit anti-Human DCUN1D1 Polyclonal Antibody | anti-DCUN1D1 antibody
Anti-DCUN1D1 Antibody, Rabbit Polyclonal
Gene Names
DCUN1D1; RP42; SCRO; Tes3; DCNL1; SCCRO; DCUN1L1
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
DCUN1D1, Antibody; Anti-DCUN1D1 Antibody, Rabbit Polyclonal; DCUN1D1/SCCRO Antibody, Rabbit PAb, Antigen Affinity Purified; DCN1, defective in cullin neddylation 1, domain containing 1; Anti-DCNL1 Antibody; Anti-DCUN1L1 Antibody; Anti-RP42 Antibody; Anti-SCCRO Antibody; Anti-SCRO Antibody; Anti-Tes3 Antibody; anti-DCUN1D1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DCUN1D1/SCCRO
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-DCUN1D1 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human DCUN1D1/SCCRO protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human DCUN1D1/SCCRO (rh DCUN1D1/SCCRO). DCUN1D1/SCCRO specific IgG was purified by Human DCUN1D1/SCCRO affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-DCUN1D1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:30 kDa)
WB (Western Blot)
(Anti-DCUN1D1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:30 kDa)
IP (Immunoprecipitation)
(DCUN1D1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-DCUN1D1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DCUN1D1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 26 kDa)
IP (Immunoprecipitation)
(DCUN1D1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-DCUN1D1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DCUN1D1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 26 kDa)
Related Product Information for anti-DCUN1D1 antibody
DCUN1D1, also known as SCCRO, is part of an E3 ubiquitin ligase complex for neddylation. DCUN1D1 functions to recruit charged E2 and is involved in the release of inhibitory effects of CAND1 on cullin-RING ligase E3 complex assembly and activity. DCUN1D1 binds to the components of the neddylation pathway (Cullin-ROC1, Ubc12, and CAND1) and augments but is not required for cullin neddylation in reactions using purified recombinant proteins. DCUN1D1 also recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation in purified protein assays. DCUN1D1 also acts as am ncogene facilitating malignant transformation and carcinogenic progression.
References
Heir P. et al., 2013, Mol Cell Biol. 33 (8): 1621-31. Kurz T. et al., 2005, Nature. 435 (7046): 1257-61. Kim AY. et al., 2008, J Biol Chem. 283 (48): 33211-20.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,124 Da
NCBI Official Full Name
DCN1-like protein 1
NCBI Official Synonym Full Names
DCN1, defective in cullin neddylation 1, domain containing 1
NCBI Official Symbol
DCUN1D1
NCBI Official Synonym Symbols
RP42; SCRO; Tes3; DCNL1; SCCRO; DCUN1L1
NCBI Protein Information
DCN1-like protein 1; DCUN1 domain-containing protein 1; RP42 homolog; defective in cullin neddylation protein 1-like protein 1; squamous cell carcinoma related oncogene; squamous cell carcinoma-related oncogene
UniProt Protein Name
DCN1-like protein 1
UniProt Gene Name
DCUN1D1
UniProt Synonym Gene Names
DCUN1L1; RP42; SCCRO
UniProt Entry Name
DCNL1_HUMAN
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Product Notes
The DCUN1D1 dcun1d1 (Catalog #AAA255804) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DCUN1D1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DCUN1D1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DCUN1D1 dcun1d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCUN1D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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