FCM/FACS (Flow Cytometry)
(DDIT3 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human DDIT3 Polyclonal Antibody | anti-DDIT3 antibody
DDIT3 Antibody (Center)
Gene Names
DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DDIT3, Antibody; DDIT3 Antibody (Center); DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153; DDIT3; CHOP10; GADD153; anti-DDIT3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DDIT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the Central region of human DDIT3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
82-110
Sequence Length
192
Applicable Applications for anti-DDIT3 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(DDIT3 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(DDIT3 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(DDIT3 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow).)
WB (Western Blot)
(DDIT3 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow).)
Related Product Information for anti-DDIT3 antibody
This gene encodes a member of the CCAAT/enhancer-binding
protein (C/EBP) family of transcription factors. The protein
functions as a dominant-negative inhibitor by forming heterodimers
with other C/EBP members, such as C/EBP and LAP (liver activator
protein), and preventing their DNA binding activity. The protein is
implicated in adipogenesis and erythropoiesis, is activated by
endoplasmic reticulum stress, and promotes apoptosis. Fusion of
this gene and FUS on chromosome 16 or EWSR1 on chromosome 22
induced by translocation generates chimeric proteins in myxoid
liposarcomas or Ewing sarcoma. Multiple alternatively spliced
transcript variants encoding two isoforms with different length
have been identified.
protein (C/EBP) family of transcription factors. The protein
functions as a dominant-negative inhibitor by forming heterodimers
with other C/EBP members, such as C/EBP and LAP (liver activator
protein), and preventing their DNA binding activity. The protein is
implicated in adipogenesis and erythropoiesis, is activated by
endoplasmic reticulum stress, and promotes apoptosis. Fusion of
this gene and FUS on chromosome 16 or EWSR1 on chromosome 22
induced by translocation generates chimeric proteins in myxoid
liposarcomas or Ewing sarcoma. Multiple alternatively spliced
transcript variants encoding two isoforms with different length
have been identified.
Product Categories/Family for anti-DDIT3 antibody
References
Park, S.H., et al. J. Immunol. 185(9):5522-5530(2010)
Goodall, J.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(41):17698-17703(2010)
Zhang, H.M., et al. J. Virol. 84(17):8446-8459(2010)
Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010) :
Wang, Y.L., et al. J. Exp. Clin. Cancer Res. 29, 54 (2010) :
Goodall, J.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(41):17698-17703(2010)
Zhang, H.M., et al. J. Virol. 84(17):8446-8459(2010)
Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010) :
Wang, Y.L., et al. J. Exp. Clin. Cancer Res. 29, 54 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19175
NCBI Official Full Name
DNA damage-inducible transcript 3 protein isoform 1
NCBI Official Synonym Full Names
DNA-damage-inducible transcript 3
NCBI Official Symbol
DDIT3
NCBI Official Synonym Symbols
CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
NCBI Protein Information
DNA damage-inducible transcript 3 protein
UniProt Protein Name
DNA damage-inducible transcript 3 protein
UniProt Gene Name
DDIT3
UniProt Synonym Gene Names
CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
UniProt Entry Name
DDIT3_HUMAN
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Product Notes
The DDIT3 ddit3 (Catalog #AAA287278) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 82-110. The DDIT3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDIT3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DDIT3 ddit3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDIT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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