Rabbit DDR1 Polyclonal Antibody | anti-DDR1 antibody

DDR1 Antibody (N-term)

Cat. Num. AAA287046

• Gene Names: DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
• Reactivity: Human, Rat (Predicted Reactivity: Mouse)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DDR1, Antibody; DDR1 Antibody (N-term); Epithelial discoidin domain-containing receptor 1; Epithelial discoidin domain receptor 1; CD167 antigen-like family member A; Cell adhesion kinase; Discoidin receptor tyrosine kinase; HGK2; Mammary carcinoma kinase 10; MCK-10; Protein-tyrosine kinase 3A; Protein-tyrosine kinase RTK-6; TRK E; Tyrosine kinase DDR; Tyrosine-protein kinase CAK; CD167a; DDR1; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; anti-DDR1 antibody

• Host: Rabbit
• Reactivity: Human, Rat (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This DDR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-47 amino acids from the N-terminal region of human DDR1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 17-47
• Sequence Length: 894

• Applicable Applications for anti-DDR1 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
• Cellular Location: [Isoform 1]: Cell membrane; Single-pass type I membrane protein [Isoform 3]: Secreted.
• Tissue Location: Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level) Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Overlay histogram showing MCF-7 cells stained with AAA287046 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA287046, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x106 cells) used under the same conditions. Acquisition of >10,000 events was performed.)

IHC (Immunohistochemistry)

(DDR1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DDR1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Anti-DDR1 Antibody (N-term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-DDR1 Antibody (N-term) at 1:2000 dilution + rat brain lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Western blot analysis of anti-DDR1 Antibody (N-term) Pab in placenta lysate. DDR1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-DDR1 antibody

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. MCK10 is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. The gene is located on chromosome 6p21.3 in proximity to several HLA class I genes.

Product Categories/Family for anti-DDR1 antibody

Cancer; Cardiovascular; Neuroscience; Signal transduction

References

Vogel, W., et al., Mol. Cell 1(1):13-23 (1997). Playford, M.P., et al., Genome Res. 6(7):620-627 (1996). Perez, J.L., et al., Oncogene 12(7):1469-1477 (1996). Valent, A., et al., Hum. Genet. 98(1):12-15 (1996). Edelhoff, S., et al., Genomics 25(1):309-311 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 321400059
• NCBI GeneID: 780
• NCBI Accession #: NP_001189450.1; NP_001189451.1; NP_001189452.1; NP_001945.3; NP_054699.2; NP_054700.2
• NCBI GenBank Nucleotide #: NM_001202521.1
• UniProt Accession #: Q08345; Q14196; Q16562; Q2L6H3; Q4LE50; Q5ST11; Q5ST12; Q6NSK4; Q9UD35; B5A975; B5A976; B7Z2K0
• NCBI Official Full Name: epithelial discoidin domain-containing receptor 1 isoform 4
• NCBI Official Synonym Full Names: discoidin domain receptor tyrosine kinase 1
• NCBI Official Symbol: DDR1
• NCBI Official Synonym Symbols: CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
• NCBI Protein Information: epithelial discoidin domain-containing receptor 1
• UniProt Protein Name: Epithelial discoidin domain-containing receptor 1
• UniProt Gene Name: DDR1
• UniProt Synonym Gene Names: CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; Epithelial discoidin domain receptor 1; MCK-10
• UniProt Entry Name: DDR1_HUMAN