WB (Western Blot)
(Detection of human DDX24 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DDX24 antibody BL3139 (Cat. No. AAA211095) used for WB at 0.04 ug (A) and 1 ug/ml (B) and used for immunoprecipitation at 3 ug/mg lysate (B). DDX24 was also immunoprecipitated by rabbit anti-DDX24 antibody BL3136 (Cat. No. and BL3138 (Cat. No. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
Rabbit anti-Human DDX24 Polyclonal Antibody | anti-DDX24 antibody
Rabbit anti-DDX24 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DDX24, Antibody; Rabbit anti-DDX24 Antibody, Affinity Purified; S. cerevisiae CHL1-like helicase; DEAD (Asp-Glu-Ala-Asp) box helicase 24; DEAD (Asp-Glu-Ala-Asp) box polypeptide 24; DEAD box protein 24; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24; ATP-dependent RNA helicase DDX24; DEAD-box helicase 24; anti-DDX24 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DDX24 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 809 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DDX24 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DDX24 antibody BL3139 (Cat. No. AAA211095) used for WB at 0.04 ug (A) and 1 ug/ml (B) and used for immunoprecipitation at 3 ug/mg lysate (B). DDX24 was also immunoprecipitated by rabbit anti-DDX24 antibody BL3136 (Cat. No. and BL3138 (Cat. No. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human DDX24 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DDX24 antibody BL3139 (Cat. No. AAA211095) used for WB at 0.04 ug (A) and 1 ug/ml (B) and used for immunoprecipitation at 3 ug/mg lysate (B). DDX24 was also immunoprecipitated by rabbit anti-DDX24 antibody BL3136 (Cat. No. and BL3138 (Cat. No. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human DDX24 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DDX24 (Cat. No. AAA211095 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DDX24 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DDX24 (Cat. No. AAA211095 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-DDX24 antibody
DDX24 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The specific function of DDX24 has not been elucidated.
Product Categories/Family for anti-DDX24 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
96,332 Da
NCBI Official Full Name
ATP-dependent RNA helicase DDX24
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
NCBI Official Symbol
DDX24
NCBI Protein Information
ATP-dependent RNA helicase DDX24; DEAD box protein 24; S. cerevisiae CHL1-like helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24
UniProt Protein Name
ATP-dependent RNA helicase DDX24
UniProt Gene Name
DDX24
UniProt Entry Name
DDX24_HUMAN
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Product Notes
The DDX24 ddx24 (Catalog #AAA211095) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DDX24 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDX24 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DDX24 ddx24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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