WB (Western Blot)
(DDX4 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDX4 antibody detected the DDX4 protein (arrow).)
Rabbit DDX4 Polyclonal Antibody | anti-DDX4 antibody
DDX4 Antibody (N-term)
Gene Names
DDX4; VASA
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DDX4, Antibody; DDX4 Antibody (N-term); Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog; DDX4; VASA; anti-DDX4 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-131 amino acids from the N-terminal region of human DDX4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
103-131
Sequence Length
690
Applicable Applications for anti-DDX4 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(DDX4 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDX4 antibody detected the DDX4 protein (arrow).)
WB (Western Blot)
(DDX4 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDX4 antibody detected the DDX4 protein (arrow).)
WB (Western Blot)
(DDX4 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DDX4 antibody detected the DDX4 protein (arrow).)
WB (Western Blot)
(DDX4 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DDX4 antibody detected the DDX4 protein (arrow).)
Related Product Information for anti-DDX4 antibody
DEAD box proteins, characterized by the conserved motif
Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
implicated in a number of cellular processes involving alteration
of RNA secondary structure such as translation initiation, nuclear
and mitochondrial splicing, and ribosome and spliceosome assembly.
Based on their distribution patterns, some members of this family
are believed to be involved in embryogenesis, spermatogenesis, and
cellular growth and division. This gene encodes a DEAD box protein,
which is a homolog of VASA proteins in Drosophila and several other
species. The gene is specifically expressed in the germ cell
lineage in both sexes and functions in germ cell development.
Multiple transcript variants encoding different isoforms have been
found for this gene.
Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
implicated in a number of cellular processes involving alteration
of RNA secondary structure such as translation initiation, nuclear
and mitochondrial splicing, and ribosome and spliceosome assembly.
Based on their distribution patterns, some members of this family
are believed to be involved in embryogenesis, spermatogenesis, and
cellular growth and division. This gene encodes a DEAD box protein,
which is a homolog of VASA proteins in Drosophila and several other
species. The gene is specifically expressed in the germ cell
lineage in both sexes and functions in germ cell development.
Multiple transcript variants encoding different isoforms have been
found for this gene.
Product Categories/Family for anti-DDX4 antibody
References
Tilgner, K., et al. Stem Cells 28(1):84-92(2010)
Sugimoto, K., et al. J. Hum. Genet. 54(8):450-456(2009)
Hashimoto, H., et al. Gynecol. Oncol. 111(2):312-319(2008)
Albamonte, M.S., et al. Hum. Reprod. 23(8):1895-1901(2008)
Guo, X., et al. Asian J. Androl. 9(3):339-344(2007)
Sugimoto, K., et al. J. Hum. Genet. 54(8):450-456(2009)
Hashimoto, H., et al. Gynecol. Oncol. 111(2):312-319(2008)
Albamonte, M.S., et al. Hum. Reprod. 23(8):1895-1901(2008)
Guo, X., et al. Asian J. Androl. 9(3):339-344(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79308
NCBI Official Full Name
probable ATP-dependent RNA helicase DDX4 isoform 2
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
NCBI Official Symbol
DDX4
NCBI Official Synonym Symbols
VASA
NCBI Protein Information
probable ATP-dependent RNA helicase DDX4
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX4
UniProt Gene Name
DDX4
UniProt Synonym Gene Names
VASA
UniProt Entry Name
DDX4_HUMAN
Similar Products
Product Notes
The DDX4 ddx4 (Catalog #AAA290268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 103-131. The DDX4 Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's DDX4 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DDX4 ddx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.