Rabbit anti-Human DDX58 Polyclonal Antibody | anti-DDX58 antibody

DDX58 Antibody

Cat. Num. AAA304040

• Gene Names: DDX58; RIGI; RIG-I; RLR-1; SGMRT2
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: DDX58, Antibody; DDX58 Antibody; DDX58; DKFZp434J1111; DKFZp686N19181; FLJ13599; RIG-I; anti-DDX58 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total DDX58 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 925

• Applicable Applications for anti-DDX58 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Peptide
• Immunogen Description: A synthetic peptide of human DDX58.
• Target Name: DDX58
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of MCF-7 cell using DDX58 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human rectal cancer using DDX58 antibody at dilution of 1:200 (200x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using DDX58 antibody.)

Related Product Information for anti-DDX58 antibody

Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4,5). Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5'-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-kB, TBK1/IKKeta, and IRF-3/IRF-7 (12-15).

Product Categories/Family for anti-DDX58 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 27881482
• NCBI GeneID: 23586
• NCBI Accession #: NP_055129.2
• NCBI GenBank Nucleotide #: NM_014314.3
• UniProt Accession #: O95786; Q5HYE1; Q5VYT1; Q9NT04; A2RU81
• Molecular Weight: 101,377 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX58
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• NCBI Official Symbol: DDX58
• NCBI Official Synonym Symbols: RIGI; RIG-I; RLR-1; SGMRT2
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX58
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX58
• UniProt Gene Name: DDX58
• UniProt Synonym Gene Names: RLR-1; RIG-1; RIG-I
• UniProt Entry Name: DDX58_HUMAN