FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCIH460 cells using Anti-DENND1B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
Rabbit anti-Human DENND1B Polyclonal Antibody | anti-DENND1B antibody
Anti-DENND1B Antibody
Gene Names
DENND1B; FAM31B; C1ORF18; C1orf218
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
DENND1B, Antibody; Anti-DENND1B Antibody; anti-DENND1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of DENND1B protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-DENND1B antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human DENND1B. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCIH460 cells using Anti-DENND1B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCIH460 cells using Anti-DENND1B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of DENND1B staining in human lymph formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of DENND1B staining in human lymph formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of DENND1B expression in MOLT4 (A) whole cell lysates. (Predicted band size: 86 kD; Observed band size: 48 kD))
WB (Western Blot)
(Western blot analysis of DENND1B expression in MOLT4 (A) whole cell lysates. (Predicted band size: 86 kD; Observed band size: 48 kD))
Related Product Information for anti-DENND1B antibody
Rabbit polyclonal antibody to DENND1B
Product Categories/Family for anti-DENND1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86,552 Da
NCBI Official Full Name
DENN domain-containing protein 1B isoform 3
NCBI Official Synonym Full Names
DENN/MADD domain containing 1B
NCBI Official Symbol
DENND1B
NCBI Official Synonym Symbols
FAM31B; C1ORF18; C1orf218
NCBI Protein Information
DENN domain-containing protein 1B; connecdenn 2; family with sequence similarity 31, member B
UniProt Protein Name
DENN domain-containing protein 1B
UniProt Gene Name
DENND1B
UniProt Synonym Gene Names
C1orf218; FAM31B
UniProt Entry Name
DEN1B_HUMAN
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Product Notes
The DENND1B dennd1b (Catalog #AAA265265) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DENND1B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DENND1B can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DENND1B dennd1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DENND1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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