WB (Western Blot)
(Sample:HepG2 Cell (Human) Lysate at 30 ugA431 Cell (Human) Lysate at 30 ugPrimary: Anti-DEPDC1B/BRCC3 (bs-14278R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62kDObserved band size: 62kD)
Rabbit anti-Rat, Cow DEPDC1B/BRCC3 Polyclonal Antibody | anti-DEPDC1B/BRCC3 antibody
DEPDC1B/BRCC3 Polyclonal Antibody
Gene Names
DEPDC1B; XTP1; BRCC3
Reactivity
Rat, Cow
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DEPDC1B/BRCC3, Antibody; DEPDC1B/BRCC3 Polyclonal Antibody; DEP domain-containing protein 1B; HBV X-transactivated gene 8 protein; HBV XAg-transactivated protein 8; DEPDC1B; XTP8; anti-DEPDC1B/BRCC3 antibody
Host
Rabbit
Reactivity
Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-DEPDC1B/BRCC3 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Sample:HepG2 Cell (Human) Lysate at 30 ugA431 Cell (Human) Lysate at 30 ugPrimary: Anti-DEPDC1B/BRCC3 (bs-14278R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62kDObserved band size: 62kD)
WB (Western Blot)
(Sample:HepG2 Cell (Human) Lysate at 30 ugA431 Cell (Human) Lysate at 30 ugPrimary: Anti-DEPDC1B/BRCC3 (bs-14278R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62kDObserved band size: 62kD)
IHC (Immunohistochemistry)
(Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-BRCC3 Polyclonal Antibody, Unconjugated(bs-14278R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohistochemistry)
(Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-BRCC3 Polyclonal Antibody, Unconjugated(bs-14278R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 58 kDa
Observed: 57 kDa
Observed: 57 kDa
NCBI Official Full Name
DEP domain-containing protein 1B isoform 1
NCBI Official Synonym Full Names
DEP domain containing 1B
NCBI Official Symbol
DEPDC1B
NCBI Official Synonym Symbols
XTP1; BRCC3
NCBI Protein Information
DEP domain-containing protein 1B
UniProt Protein Name
DEP domain-containing protein 1B
UniProt Gene Name
DEPDC1B
UniProt Synonym Gene Names
XTP8
UniProt Entry Name
DEP1B_HUMAN
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Product Notes
The DEPDC1B/BRCC3 depdc1b (Catalog #AAA291545) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DEPDC1B/BRCC3 Polyclonal Antibody reacts with Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's DEPDC1B/BRCC3 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DEPDC1B/BRCC3 depdc1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEPDC1B/BRCC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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