Rabbit DEPTOR Polyclonal Antibody | anti-DEPTOR antibody

DEPTOR Antibody

Cat. Num. AAA40587

• Gene Names: DEPTOR; DEP.6; DEPDC6
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: DEPTOR antibody is affinity chromatography purified via peptide column.
• Synonyms: DEPTOR, Antibody; DEPTOR Antibody; DEPTOR; DEP.6; DEPDC6; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; DEP domain containing MTOR-interacting protein; anti-DEPTOR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms.
• Purity/Purification: DEPTOR antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 409

• Applicable Applications for anti-DEPTOR antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR.
• Buffer: DEPTOR Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DEPTOR antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

IHC (Immunohiostchemistry)

(Immunohistochemistry of DEPTOR in mouse liver tissue with DEPTOR antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 μg/ml.)

Related Product Information for anti-DEPTOR antibody

DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).

NCBI and Uniprot Product Information

• NCBI GI #: 189571664
• NCBI GeneID: 64798
• NCBI Accession #: NP_073620
• NCBI GenBank Nucleotide #: NM_022783.3
• UniProt Accession #: Q8TB45; Q96EQ1; Q9H0R7; Q9H894; Q9HA07; B2RCL9; B4DN97; E7EV87
• Molecular Weight: Predicted: 45 kDa; ; Observed: 42 kDa
• NCBI Official Full Name: DEP domain-containing mTOR-interacting protein isoform 1
• NCBI Official Synonym Full Names: DEP domain containing MTOR-interacting protein
• NCBI Official Symbol: DEPTOR
• NCBI Official Synonym Symbols: DEP.6; DEPDC6
• NCBI Protein Information: DEP domain-containing mTOR-interacting protein; DEP domain containing 6; DEP domain-containing protein 6; DEP domain containing mTOR interacting protein
• UniProt Protein Name: DEP domain-containing mTOR-interacting protein
• UniProt Gene Name: DEPTOR
• UniProt Synonym Gene Names: DEPDC6
• UniProt Entry Name: DPTOR_HUMAN