Rabbit anti-Human DFNB31 Polyclonal Antibody | anti-DFNB31 antibody

DFNB31 Antibody (Center)

Cat. Num. AAA285831

• Gene Names: DFNB31; WI; WHRN; CIP98; USH2D; PDZD7B
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: DFNB31, Antibody; DFNB31 Antibody (Center); Whirlin; Autosomal recessive deafness type 31 protein; DFNB31; KIAA1526; WHRN; anti-DFNB31 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DFNB31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-406 amino acids from the Central region of human DFNB31.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 378-406
• Sequence Length: 524

• Applicable Applications for anti-DFNB31 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(DFNB31 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DFNB31 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of lysate from human kidney tissue lysate, using DFNB31 Antibody (Center). AAA285831 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

Related Product Information for anti-DFNB31 antibody

This gene is thought to function in the organization and stabilization of sterocilia elongation and actin cystoskeletal assembly, based on studies of the related mouse gene. Mutations in this gene have been associated with autosomal recessive non-syndromic deafness and Usher Syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Cellular Location: Cytoplasm {ECO:0000250|UniProtKB:Q80VW5}. Cell projection, stereocilium {ECO:0000250|UniProtKB:Q80VW5}. Cell projection, growth cone {ECO:0000250|UniProtKB:Q80VW5}. Photoreceptor inner segment {ECO:0000250|UniProtKB:Q80VW5}. Cell junction, synapse {ECO:0000250|UniProtKB:Q810W9}. Note=Detected at the level of stereocilia in inner and outer hair cells of the cochlea and vestibule Localizes to both tip and ankle-link stereocilia regions. Colocalizes with the growing ends of actin filaments. Colocalizes with MPP1 in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC). In photoreceptors, localizes at a plasma membrane microdomain in the apical inner segment that surrounds the connecting cilia called periciliary membrane complex. {ECO:0000250|UniProtKB:Q80VW5, ECO:0000250|UniProtKB:Q810W9, ECO:0000269|PubMed:17584769}

Product Categories/Family for anti-DFNB31 antibody

Neuroscience

References

Letra, A., et al. Am. J. Med. Genet. A 152A (7), 1701-1710 (2010) :
Secolin, R., et al. Psychiatr. Genet. 20(3):126-129(2010)
Aller, E., et al. Mol. Vis. 16, 495-500 (2010) :
Toiyama, Y., et al. Int. J. Oncol. 35(4):709-715(2009)
Gosens, I., et al. Hum. Mol. Genet. 16(16):1993-2003(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 290746378
• NCBI GeneID: 25861
• NCBI Accession #: NP_001077354.2; NP_001166896.1; NP_056219.3
• NCBI GenBank Nucleotide #: NM_001083885.2
• UniProt Accession #: Q9P202; Q5TAU9; Q5TAV0; Q5TAV1; Q5TAV2; Q96MZ9; Q9H9F4; Q9UFZ3; A5PKU1; A5PKZ9
• Molecular Weight: Calculated MW: 96558
• NCBI Official Full Name: whirlin isoform 2
• NCBI Official Synonym Full Names: deafness, autosomal recessive 31
• NCBI Official Symbol: DFNB31
• NCBI Official Synonym Symbols: WI; WHRN; CIP98; USH2D; PDZD7B
• NCBI Protein Information: whirlin
• UniProt Protein Name: Whirlin
• UniProt Gene Name: DFNB31
• UniProt Synonym Gene Names: KIAA1526; WHRN
• UniProt Entry Name: WHRN_HUMAN