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Application Data
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: DLD knockout HepG2 cell lysate; Predicted MW: 54kd Observed MW: 58kd Primary Ab: 1ug/ml Rabbit Anti-Human DLD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human, Mouse Dihydrolipoyl Dehydrogenase (DLD) Polyclonal Antibody | anti-DLD antibody
Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)
Gene Names
DLD; E3; LAD; DLDD; DLDH; GCSL; PHE3
Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Synonyms
Dihydrolipoyl Dehydrogenase (DLD), Antibody; Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD); anti-DLD antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against DLD. It has been selected for its ability to recognize DLD in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- G ATKKSDGKID VSIEAASGGK AEVITCDVLL VCIGRRPFTK NLGLEELGIE LDPRGRIPVN TRFQTKIPNI YAIGDVVAGP MLAHKAEDEG IICVEGMAGG AVHIDYNCVP SVIYTHPEVA WVGKSEEQLK EEGIEYKVGK FPFAANSRAK TNADTDGMVK ILGQKSTDRV LGAHILGPGA GEMVNEAALA LEYGASCEDI
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- G ATKKSDGKID VSIEAASGGK AEVITCDVLL VCIGRRPFTK NLGLEELGIE LDPRGRIPVN TRFQTKIPNI YAIGDVVAGP MLAHKAEDEG IICVEGMAGG AVHIDYNCVP SVIYTHPEVA WVGKSEEQLK EEGIEYKVGK FPFAANSRAK TNADTDGMVK ILGQKSTDRV LGAHILGPGA GEMVNEAALA LEYGASCEDI
Sequence Length
630
Applicable Applications for anti-DLD antibody
WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Recombinant DLD (Gly280~His487) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Application Data
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: DLD knockout HepG2 cell lysate; Predicted MW: 54kd Observed MW: 58kd Primary Ab: 1ug/ml Rabbit Anti-Human DLD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Application Data
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: DLD knockout HepG2 cell lysate; Predicted MW: 54kd Observed MW: 58kd Primary Ab: 1ug/ml Rabbit Anti-Human DLD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Stomach Tissue))
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Stomach Tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human DLD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human DLD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-DLD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,283 Da
NCBI Official Full Name
dihydrolipoyl dehydrogenase, mitochondrial isoform 1
NCBI Official Synonym Full Names
dihydrolipoamide dehydrogenase
NCBI Official Symbol
DLD
NCBI Official Synonym Symbols
E3; LAD; DLDD; DLDH; GCSL; PHE3
NCBI Protein Information
dihydrolipoyl dehydrogenase, mitochondrial
UniProt Protein Name
Dihydrolipoyl dehydrogenase, mitochondrial
UniProt Gene Name
DLD
UniProt Synonym Gene Names
GCSL; LAD; PHE3
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Product Notes
The DLD dld (Catalog #AAA135082) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Dihydrolipoyl Dehydrogenase (DLD) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the DLD dld for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- G ATKKSDGKID VSIEAASGGK AEVITCDVLL VCIGRRPFTK NLGLEELGIE LDPRGRIPVN TRFQTKIPNI YAIGDVVAGP MLAHKAEDEG IICVEGMAGG AVHIDYNCVP SVIYTHPEVA WVGKSEEQLK EEGIEYKVGK FPFAANSRAK TNADTDGMVK ILGQKSTDRV LGAHILGPGA GEMVNEAALA LEYGASCEDI. It is sometimes possible for the material contained within the vial of "Dihydrolipoyl Dehydrogenase (DLD), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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