IHC (Immunohiostchemistry)
(AAA327508 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
Rabbit DIO3 Polyclonal Antibody | anti-DIO3 antibody
DIO3 Antibody
Gene Names
DIO3; D3; 5DIII; TXDI3; DIOIII
Reactivity
Human , Mouse , Rat , Monkey
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Synonyms
DIO3, Antibody; DIO3 Antibody; 5DIII; D3; Deiodinase iodothyronine type 3; Deiodinase iodothyronine type III; Dio 3; Dio3; DIOIII; IOD3_HUMAN; Iodothyronine deiodinase placental type; MGC124117; MGC124118; Thyroxine deiodinase type III; Thyroxine deiodinase type III (selenoprotein); TXDI3; Type 3 DI; Type 3 iodothyronine selenodeiodinase; Type III 5' deiodinase; Type III iodothyronine deiodinase; Type-III 5''-deiodinase; anti-DIO3 antibody
Host
Rabbit
Reactivity
Human , Mouse , Rat , Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
DIO3 antibody detects endogenous levels of total DIO3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
304
Applicable Applications for anti-DIO3 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human DIO3, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA327508 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
IHC (Immunohiostchemistry)
(AAA327508 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
WB (Western Blot)
(Western blot analysis of DIO3 using COS7 whole cell lysates)
WB (Western Blot)
(Western blot analysis of DIO3 using COS7 whole cell lysates)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31 kDa
NCBI Official Full Name
thyroxine 5-deiodinase
NCBI Official Synonym Full Names
iodothyronine deiodinase 3
NCBI Official Symbol
DIO3
NCBI Official Synonym Symbols
D3; 5DIII; TXDI3; DIOIII
NCBI Protein Information
thyroxine 5-deiodinase
UniProt Protein Name
Thyroxine 5-deiodinase
UniProt Gene Name
DIO3
UniProt Synonym Gene Names
ITDI3; TXDI3
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Product Notes
The DIO3 dio3 (Catalog #AAA327508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DIO3 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's DIO3 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DIO3 dio3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIO3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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