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product-image-AAA118644_ChIP11.jpg ChIP (Chromatin Immunoprecipitation) (Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-POLR3A or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the tRNA-Leu Anti-Codon (TAG) promoter.)

Rabbit anti-Human DNA-directed RNA polymerase III subunit RPC1 Polyclonal Antibody | anti-POLR3A antibody

Rabbit anti-human DNA-directed RNA polymerase III subunit RPC1 polyclonal Antibody(POLR3A)

Gene Names
POLR3A; ADDH; HLD7; RPC1; RPC155; hRPC155
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, ELISA
Purity
>95%, Protein G purified
Synonyms
DNA-directed RNA polymerase III subunit RPC1, Antibody; Rabbit anti-human DNA-directed RNA polymerase III subunit RPC1 polyclonal Antibody(POLR3A); DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; RNA polymerase III subunit C160; RPC155; POLR3A; anti-POLR3A antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Sequence Length
1390
Applicable Applications for anti-POLR3A antibody
ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), ELISA
Conjugate
Non-conjugated
Immunogen Species
Homo sapiens (Human)
Research Area
Epigenetics and Nuclear Signaling
Target Names
POLR3A
Immunogen
Recombinant human DNA-directed RNA polymerase III subunit RPC1 protein (392-632AA)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-107058 / sc-107059 / sc-292119
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid repeated freeze.

ChIP (Chromatin Immunoprecipitation)

(Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-POLR3A or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the tRNA-Leu Anti-Codon (TAG) promoter.)

product-image-AAA118644_ChIP11.jpg ChIP (Chromatin Immunoprecipitation) (Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-POLR3A or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the tRNA-Leu Anti-Codon (TAG) promoter.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human small intestine tissue using AAA118644 at dilution 1:100)

product-image-AAA118644_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human small intestine tissue using AAA118644 at dilution 1:100)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human testis using AAA118644 at dilution 1:100)

product-image-AAA118644_IHC15.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human testis using AAA118644 at dilution 1:100)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA-directed RNA polymerase III subunit RPC1
NCBI Official Synonym Full Names
polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
NCBI Official Symbol
POLR3A
NCBI Official Synonym Symbols
ADDH; HLD7; RPC1; RPC155; hRPC155
NCBI Protein Information
DNA-directed RNA polymerase III subunit RPC1
UniProt Protein Name
DNA-directed RNA polymerase III subunit RPC1
UniProt Gene Name
POLR3A
UniProt Synonym Gene Names
RNA polymerase III subunit C1; RPC155
UniProt Entry Name
RPC1_HUMAN

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Product Notes

The POLR3A polr3a (Catalog #AAA118644) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human DNA-directed RNA polymerase III subunit RPC1 polyclonal Antibody(POLR3A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA-directed RNA polymerase III subunit RPC1 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the POLR3A polr3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA-directed RNA polymerase III subunit RPC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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