IF (Immunofluorescence)
(Immunofluorescence staining of PRKDC in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PRKDC polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
Rabbit DNA-PKcs/PRKDC Polyclonal Antibody | anti-PRKDC antibody
Anti-DNA-PKcs/PRKDC Antibody, Rabbit Polyclonal
Gene Names
PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs
Reactivity
HumanPredicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
DNA-PKcs/PRKDC, Antibody; Anti-DNA-PKcs/PRKDC Antibody, Rabbit Polyclonal; PRKDC Antibody, Rabbit PAb, Antigen Affinity Purified; protein kinase, DNA-activated, catalytic polypeptide; Anti-DNA-PKcs Antibody; Anti-DNAPK Antibody; Anti-DNPK1 Antibody; Anti-HYRC Antibody; Anti-HYRC1 Antibody; Anti-IMD26 Antibody; Anti-p350 Antibody; Anti-XRCC7 Antibody; anti-PRKDC antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DNA-PKcs/PRKDC
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PRKDC antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human PRKDC
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PRKDC, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of PRKDC in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PRKDC polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of PRKDC in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PRKDC polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
IHC (Immunohistochemistry)
(Immunochemical staining of human PRKDC in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human PRKDC in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-PRKDC antibody
PRKDC encodes for DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a kinase that forms part of a complex (DNA-dependent protein kinase [DNA-PK]) crucial for DNA double-strand break repair and V(D)J recombination. PRKDC expression was significantly increased in breast cancer tissue samples compared with NATs. High PRKDC expression was associated with higher tumor grade (P=0.001), positive lymph node metastasis (P=0.0357) and chemoresistance (P=0.0006). Furthermore, PRKDC expression was significantly correlated with OS in breast cancer patients with (0.0101) or without (P=0.0216) receiving chemotherapy. Collectively, PRKDC is a prognostic biomarker for chemoresistance in breast cancer patients.
Product Categories/Family for anti-PRKDC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
469,089 Da
NCBI Official Full Name
DNA-dependent protein kinase catalytic subunit isoform 2
NCBI Official Synonym Full Names
protein kinase, DNA-activated, catalytic polypeptide
NCBI Official Symbol
PRKDC
NCBI Official Synonym Symbols
HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs
NCBI Protein Information
DNA-dependent protein kinase catalytic subunit; p460; DNA-PK catalytic subunit; hyper-radiosensitivity of murine scid mutation, complementing 1
UniProt Protein Name
DNA-dependent protein kinase catalytic subunit
UniProt Gene Name
PRKDC
UniProt Synonym Gene Names
HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs
UniProt Entry Name
PRKDC_HUMAN
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Product Notes
The PRKDC prkdc (Catalog #AAA254443) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DNA-PKcs/PRKDC Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's DNA-PKcs/PRKDC can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PRKDC prkdc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA-PKcs/PRKDC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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