FCM/FACS (Flow Cytometry)
(DNAJC6 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse DNAJC6 Polyclonal Antibody | anti-DNAJC6 antibody
DNAJC6 Antibody (Center)
Gene Names
DNAJC6; DJC6; PARK19
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DNAJC6, Antibody; DNAJC6 Antibody (Center); Putative tyrosine-protein phosphatase auxilin; DnaJ homolog subfamily C member 6; DNAJC6; KIAA0473; anti-DNAJC6 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DNAJC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-281 amino acids from the Central region of human DNAJC6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
254-281
Sequence Length
970
Applicable Applications for anti-DNAJC6 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(DNAJC6 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(DNAJC6 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(DNAJC6 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNAJC6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(DNAJC6 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNAJC6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(DNAJC6 Antibody (Center) western blot analysis in K562(lane 1),HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).This demonstrates the DNAJC6 antibody detected the DNAJC6 protein (arrow).)
WB (Western Blot)
(DNAJC6 Antibody (Center) western blot analysis in K562(lane 1),HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).This demonstrates the DNAJC6 antibody detected the DNAJC6 protein (arrow).)
Related Product Information for anti-DNAJC6 antibody
DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain
References
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Hirst, J., et al. Traffic 9(8):1354-1371(2008)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Hirst, J., et al. Traffic 9(8):1354-1371(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99997
NCBI Official Full Name
putative tyrosine-protein phosphatase auxilin isoform 1
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily C, member 6
NCBI Official Symbol
DNAJC6
NCBI Official Synonym Symbols
DJC6; PARK19
NCBI Protein Information
putative tyrosine-protein phosphatase auxilin
UniProt Protein Name
Putative tyrosine-protein phosphatase auxilin
UniProt Gene Name
DNAJC6
UniProt Synonym Gene Names
KIAA0473
UniProt Entry Name
AUXI_HUMAN
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Product Notes
The DNAJC6 dnajc6 (Catalog #AAA288345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 254-281. The DNAJC6 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJC6 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DNAJC6 dnajc6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJC6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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