IF (Immunofluorescence)
(Detection of DNMT1 by immunofluorescence. U2OS cells were stained with DNMT1 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: DNMT1 antibody staining. Right panel: merge.)
Rabbit anti-Human DNMT1 Polyclonal Antibody | anti-DNMT1 antibody
DNMT1 antibody (pAb)
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Protein A Chromatography
Synonyms
DNMT1, Antibody; DNMT1 antibody (pAb); AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; anti-DNMT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-DNMT1 antibody
WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence)
Immunogen
This DNMT1 antibody was raised against a recombinant protein corresponding to amino acids 1-110 of human DNMT1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
IF (Immunofluorescence)
(Detection of DNMT1 by immunofluorescence. U2OS cells were stained with DNMT1 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: DNMT1 antibody staining. Right panel: merge.)
IF (Immunofluorescence)
(Detection of DNMT1 by immunofluorescence. U2OS cells were stained with DNMT1 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: DNMT1 antibody staining. Right panel: merge.)
WB (Western Blot)
(DNMT1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug per lane) probed with DNMT1 antibody (pAb) at a dilution of 0.5 ug/ml.)
WB (Western Blot)
(DNMT1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug per lane) probed with DNMT1 antibody (pAb) at a dilution of 0.5 ug/ml.)
WB (Western Blot)
(DNMT1 antibody (pAb) tested by Immunoprecipitation. 10 ug of DNMT1 antibody was used to immunoprecipitate DNMT1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the DNMT1 antibody at a dilution of 0.5 ug/ml.)
WB (Western Blot)
(DNMT1 antibody (pAb) tested by Immunoprecipitation. 10 ug of DNMT1 antibody was used to immunoprecipitate DNMT1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the DNMT1 antibody at a dilution of 0.5 ug/ml.)
Related Product Information for anti-DNMT1 antibody
Short Description: Our DNMT1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.
Background: DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.
Product Categories/Family for anti-DNMT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
144,465 Da
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 1 isoform b
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 1
NCBI Official Symbol
DNMT1
NCBI Official Synonym Symbols
AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Gene Name
DNMT1
UniProt Synonym Gene Names
AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
UniProt Entry Name
DNMT1_HUMAN
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Product Notes
The DNMT1 dnmt1 (Catalog #AAA59945) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNMT1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNMT1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence). Researchers should empirically determine the suitability of the DNMT1 dnmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNMT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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