DB (Dot Blot)
(DNMT1 monomethyl Lys142 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of DNMT1 monomethyl Lys142. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:100,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Monomethyl Lys142 peptide. Lane 2: Dimethyl lysine 142 peptide. Lane 3: Trimethyl lysine142 peptide. Lane 4: Unmodified lysine 142 peptide.)
Rabbit anti-Human DNMT1 monomethyl Lys142 Polyclonal Antibody | anti-DNMT1 antibody
DNMT1 monomethyl Lys142 antibody (pAb)
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
DNMT1 monomethyl Lys142, Antibody; DNMT1 monomethyl Lys142 antibody (pAb); AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; anti-DNMT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-DNMT1 antibody
WB (Western Blot)
Conjugation
Methylated
Immunogen
This antibody was raised against a peptide including monomethyl lysine 142 of human DNMT1.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(DNMT1 monomethyl Lys142 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of DNMT1 monomethyl Lys142. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:100,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Monomethyl Lys142 peptide. Lane 2: Dimethyl lysine 142 peptide. Lane 3: Trimethyl lysine142 peptide. Lane 4: Unmodified lysine 142 peptide.)
DB (Dot Blot)
(DNMT1 monomethyl Lys142 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of DNMT1 monomethyl Lys142. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:100,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Monomethyl Lys142 peptide. Lane 2: Dimethyl lysine 142 peptide. Lane 3: Trimethyl lysine142 peptide. Lane 4: Unmodified lysine 142 peptide.)
WB (Western Blot)
(DNMT1 monomethyl Lys142 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 ug) probed with DNMT1 monomethyl Lys142 antibody (1:500).)
WB (Western Blot)
(DNMT1 monomethyl Lys142 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 ug) probed with DNMT1 monomethyl Lys142 antibody (1:500).)
Related Product Information for anti-DNMT1 antibody
Short Description: Our DNMT1 monomethyl Lys142 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Methylation at lysine 142 by SETD7 promotes DNMT1 proteasomal degradation while phosphorylation of serine 143 via AKT blocks degradation. These modifications appear to play an important role in regulating DNMT enzyme activity and thus levels of DNA methylation.
Background: DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Methylation at lysine 142 by SETD7 promotes DNMT1 proteasomal degradation while phosphorylation of serine 143 via AKT blocks degradation. These modifications appear to play an important role in regulating DNMT enzyme activity and thus levels of DNA methylation.
Product Categories/Family for anti-DNMT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
144,465 Da
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 1 isoform a
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 1
NCBI Official Symbol
DNMT1
NCBI Official Synonym Symbols
AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA MTase HsaI; DNA methyltransferase HsaI; m.HsaI
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Gene Name
DNMT1
UniProt Synonym Gene Names
AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
UniProt Entry Name
DNMT1_HUMAN
Similar Products
Product Notes
The DNMT1 dnmt1 (Catalog #AAA60037) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNMT1 monomethyl Lys142 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNMT1 monomethyl Lys142 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the DNMT1 dnmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNMT1 monomethyl Lys142, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.