IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit DOK1 Polyclonal Antibody | anti-DOK1 antibody
DOK1 Antibody (N-term)
Gene Names
DOK1; P62DOK
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DOK1, Antibody; DOK1 Antibody (N-term); Docking protein 1; Downstream of tyrosine kinase 1; p62(dok); pp62; DOK1; anti-DOK1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human DOK1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
9-39
Sequence Length
342
Applicable Applications for anti-DOK1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of DOK1 (arrow) using DOK1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK1 gene (Lane 2).)
WB (Western Blot)
(Western blot analysis of DOK1 (arrow) using DOK1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK1 gene (Lane 2).)
WB (Western Blot)
(Western blot analysis of anti-DOK1 Pab in HL-60 cell lysate. DOK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of anti-DOK1 Pab in HL-60 cell lysate. DOK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-DOK1 antibody
DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
Product Categories/Family for anti-DOK1 antibody
References
Liang, X., et al., J. Biol. Chem. 277(16):13732-13738 (2002).
Yamakawa, N., et al., EMBO J. 21(7):1684-1694 (2002).
Hubert, P., et al., Eur. J. Immunogenet. 27(3):145-148 (2000).
Nemorin, J.G., et al., J. Biol. Chem. 275(19):14590-14597 (2000).
Nelms, K., et al., Genomics 53(2):243-245 (1998).
Yamakawa, N., et al., EMBO J. 21(7):1684-1694 (2002).
Hubert, P., et al., Eur. J. Immunogenet. 27(3):145-148 (2000).
Nemorin, J.G., et al., J. Biol. Chem. 275(19):14590-14597 (2000).
Nelms, K., et al., Genomics 53(2):243-245 (1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52392
NCBI Official Full Name
docking protein 1 isoform 2
NCBI Official Synonym Full Names
docking protein 1, 62kDa (downstream of tyrosine kinase 1)
NCBI Official Symbol
DOK1
NCBI Official Synonym Symbols
P62DOK
NCBI Protein Information
docking protein 1
UniProt Protein Name
Docking protein 1
UniProt Gene Name
DOK1
UniProt Entry Name
DOK1_HUMAN
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Product Notes
The DOK1 dok1 (Catalog #AAA286129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 9-39. The DOK1 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's DOK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DOK1 dok1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DOK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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