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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of DPP3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit DPP3 Polyclonal Antibody | anti-DPP3 antibody
DPP3 Antibody (C-term)
Gene Names
DPP3; DPPIII
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DPP3, Antibody; DPP3 Antibody (C-term); Dipeptidyl peptidase 3; Dipeptidyl aminopeptidase III; Dipeptidyl arylamidase III; Dipeptidyl peptidase III; DPP III; Enkephalinase B; DPP3; anti-DPP3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583-612 amino acids from the C-terminal region of human DPP3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.32 (varies by lot)
Sequence Positions
583-612
Sequence Length
707
Applicable Applications for anti-DPP3 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of DPP3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of DPP3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(DPP3 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(DPP3 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(DPP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DPP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(DPP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DPP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(DPP3 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DPP3 antibody detected the DPP3 protein (arrow).)
WB (Western Blot)
(DPP3 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DPP3 antibody detected the DPP3 protein (arrow).)
Related Product Information for anti-DPP3 antibody
This gene encodes a protein that is a member of the M49
family of metallopeptidases. This cytoplasmic protein binds a
single zinc ion with its zinc-binding motif (HELLGH) and has
post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro
dipeptides from the N-termini of proteins. Increased activity of
this protein is associated with endometrial and ovarian cancers.
Alternate transcriptional splice variants have been characterized.
family of metallopeptidases. This cytoplasmic protein binds a
single zinc ion with its zinc-binding motif (HELLGH) and has
post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro
dipeptides from the N-termini of proteins. Increased activity of
this protein is associated with endometrial and ovarian cancers.
Alternate transcriptional splice variants have been characterized.
Product Categories/Family for anti-DPP3 antibody
References
Shukla, A.A., et al. FEBS J. 277(8):1861-1875(2010)
Salopek-Sondi, B., et al. Biol. Chem. 389(2):163-167(2008)
Gevaert, K., et al. Proteomics 5(14):3589-3599(2005)
Abramic, M., et al. Int. J. Biochem. Cell Biol. 36(3):434-446(2004)
Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004) :
Salopek-Sondi, B., et al. Biol. Chem. 389(2):163-167(2008)
Gevaert, K., et al. Proteomics 5(14):3589-3599(2005)
Abramic, M., et al. Int. J. Biochem. Cell Biol. 36(3):434-446(2004)
Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82589
NCBI Official Full Name
dipeptidyl peptidase 3 isoform 2
NCBI Official Synonym Full Names
dipeptidyl-peptidase 3
NCBI Official Symbol
DPP3
NCBI Official Synonym Symbols
DPPIII
NCBI Protein Information
dipeptidyl peptidase 3
UniProt Protein Name
Dipeptidyl peptidase 3
UniProt Gene Name
DPP3
UniProt Synonym Gene Names
DPP III
UniProt Entry Name
DPP3_HUMAN
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Product Notes
The DPP3 dpp3 (Catalog #AAA289172) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 583-612. The DPP3 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's DPP3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DPP3 dpp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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