Rabbit DR6 Polyclonal Antibody | anti-DR6 antibody

Phospho-DR6 (Thr460) Antibody

Cat. Num. AAA328325

• Gene Names: TNFRSF21; DR6; CD358; BM-018
• Reactivity: Human, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: DR6, Antibody; Phospho-DR6 (Thr460) Antibody; TNFRSF 21; BM 018; BM018; CD358; Death receptor 6; DR 6; DR6; MGC31965; OTTHUMP00000039915; TNFR related death receptor 6; TNFRSF21; TNFRSF21 protein; TNR21_HUMAN; Tumor necrosis factor receptor superfamily member 21; Tumor necrosis factor receptor superfamily member 21 precursor; anti-DR6 antibody

• Host: Rabbit
• Reactivity: Human, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-DR6 (Thr460) Antibody detects endogenous levels of DR6 only when phosphorylated at Thr460.
• Purity/Purification: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 655

• Applicable Applications for anti-DR6 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human DR6 around the phosphorylation site of Thr460.
• Tissue Specificity: Detected in fetal spinal cord and in brain neurons, with higher levels in brain from Alzheimer disease patients (at protein level). Highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. Detected at lower levels in lung, skeletal muscle, kidney, testis, uterus, small intestine, colon, spleen, bone marrow and fetal liver. Very low levels were found in adult liver and peripheral blood leukocytes.
• Subcellular Location: Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of Phospho-DR6 (Thr460) in lysates of A549?, using Phospho-DR6 (Thr460) Antibody.)

IHC (Immunohiostchemistry)

(At 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IHC (Immunohistochemistry)

(At 1/100 staining human lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Related Product Information for anti-DR6 antibody

Description: Promotes apoptosis, possibly via a pathway that involves the activation of NF-kappa-B. Can also promote apoptosis mediated by BAX and by the release of cytochrome c from the mitochondria into the cytoplasm. Plays a role in neuronal apoptosis, including apoptosis in response to amyloid peptides derived from APP, and is required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N-terminal fragment of APP (N-APP). N-APP binds TNFRSF21; this triggers caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Negatively regulates oligodendrocyte survival, maturation and myelination. Plays a role in signaling cascades triggered by stimulation of T-cell receptors, in the adaptive immune response and in the regulation of T-cell differentiation and proliferation. Negatively regulates T-cell responses and the release of cytokines such as IL4, IL5, IL10, IL13 and IFNG by Th2 cells. Negatively regulates the production of IgG, IgM and IgM in response to antigens. May inhibit the activation of JNK in response to T-cell stimulation.

Subunit Structure: Interacts with N-APP (By similarity). Associates with TRADD. Interacts with NGFR.

NCBI and Uniprot Product Information

• NCBI GI #: 21264087
• NCBI GeneID: 27242
• UniProt Accession #: O75509
• Molecular Weight: Observed: 72 kDa; Predicted: 72 kDa
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 21
• NCBI Official Synonym Full Names: TNF receptor superfamily member 21
• NCBI Official Symbol: TNFRSF21
• NCBI Official Synonym Symbols: DR6; CD358; BM-018
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 21
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 21
• UniProt Gene Name: TNFRSF21
• UniProt Synonym Gene Names: DR6
• UniProt Entry Name: TNR21_HUMAN