WB (Western Blot)
(Detection of human Drosha by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Drosha antibody AAA211666 (lot AAA211666-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human Drosha Polyclonal Antibody | anti-Drosha antibody
Rabbit anti-Drosha Antibody, Affinity Purified
Gene Names
DROSHA; RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Drosha, Antibody; Rabbit anti-Drosha Antibody, Affinity Purified; RANSE3L; ribonuclease 3; RNASEN; RNASE3L; RNase III; RN3; ribonuclease type III, nuclear; drosha, double-stranded RNA-specific endoribonuclease; putative protein p241 which interacts with transcription factor Sp1; putative ribonuclease III; Protein Drosha; p241; nuclear RNase III Drosha; HSA242976; ETOHI2; Ribonuclease III; Ribonuclease 3; drosha ribonuclease III; anti-Drosha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-Drosha antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1324 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Drosha by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Drosha antibody AAA211666 (lot AAA211666-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human Drosha by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Drosha antibody AAA211666 (lot AAA211666-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Drosha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Drosha antibody AAA211666 (lot AAA211666-2) used for IP at 6 ug per reaction. Drosha was also immunoprecipitated by rabbit anti-Drosha antibody BL7681. For blotting immunoprecipitated Drosha, AAA211666 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Drosha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Drosha antibody AAA211666 (lot AAA211666-2) used for IP at 6 ug per reaction. Drosha was also immunoprecipitated by rabbit anti-Drosha antibody BL7681. For blotting immunoprecipitated Drosha, AAA211666 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-Drosha antibody
Drosha is one of three human RNase III enzymes (Drosha, Dicer, and Argonaute) that play a diverse role in the processing and maturation of RNA. Drosha, along with DGCR8 (DeGeorge Syndrome Chromomsomal Region 8, also known as Pasha in C. elegans and D. melanogaster) is a component of the Microprocessor complex. In this complex, Drosha functions as a double-stranded endoribonuclease critical to the first step in the processing of human microRNAs. During this step, Drosha is responsible for cleaving pri-miRNA to yield hairpin-shaped pre-miRNA for futher processing by the cytoplasmic RNase III enzyme, Dicer.
Product Categories/Family for anti-Drosha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
159,316 Da
NCBI Official Full Name
ribonuclease 3 isoform 1
NCBI Official Synonym Full Names
drosha, ribonuclease type III
NCBI Official Symbol
DROSHA
NCBI Official Synonym Symbols
RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
NCBI Protein Information
ribonuclease 3; p241; RNase III; protein Drosha; OTTHUMP00000220911; OTTHUMP00000220912; nuclear RNase III Drosha; putative ribonuclease III; ribonuclease III, nuclear; ribonuclease type III, nuclear; drosha, double-stranded RNA-specific endoribonuclease;
UniProt Protein Name
Ribonuclease 3
UniProt Gene Name
DROSHA
UniProt Synonym Gene Names
RN3; RNASE3L; RNASEN; RNase III
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Product Notes
The Drosha drosha (Catalog #AAA211666) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Drosha Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Drosha can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Drosha drosha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Drosha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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